Gene Targeting Strategy
The Col10a1 gene encodes a short-chain minor collagen of cartilage which is a direct transcriptional target of RUNX2 during chondrogenesis. In this strain, Cre recombinase expression is under the control of Col10a1 promoter. When crossed with a strain containing a loxP site-flanked sequence of interest, Cre-mediated recombination results in deletion of the flanked sequence in Col10a1 expressing cells.
Mice hemizygous for Col10a1iCre gene are viable and fertile without any physical abnormalities.
(B-EM-001) B-Col10a1-iCre mice