Cd27 (CD27 antigen)
CD27 is a TNF receptor super family
member. It is expressed on the surface of T and B cells, and its binding with CD70 provides a co-stimulatory
signal for T and B cell proliferation and for immunoglobulin production by B
cells. Treating mice with a CD27 agonist antibody enhances the anti-tumor
immune response in the MC38 colon carcinoma model.
Protein expression analysis
Fig 1. Splenocytes from both wild type (WT) C57BL/6 and homozygous B-hCD27 mice were analyzed by flow cytometry.
Mouse CD27+ T cells were detected in both WT C57BL/6 and homozygous B-hCD27 mice, while human CD27+ T cells were only detected in the homozygous B-hCD27 mice. This might be due to cross-recognition of hCD27 by anti-mCD27 antibodies.
CD27 antibody efficacy evaluation
Fig 2. Murine colon cancer MC38 cells were subcutaneously implanted into homozygote B-hCD27 mice. Mice were divided into control and treatment groups (n=5) when the tumor size was approximately 150±50 mm3. Anti-hCD27 antibody significantly inhibited tumor growth in homozygote B-hCD27 mice, suggesting that the B-hCD27 mouse model is an effective tool for in vivo hCD27 antibody efficacy studies. (A) Tumor average volume ± SEM, (B) Mice average weight ± SEM.