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Animal Models and Cell Lines

>Single Humanized Immune-Checkpoint Mice

CD27 is a TNF receptor super family member. It is expressed on the surface of T and B cells, and its binding with CD70 provides a co-stimulatory signal for T and B cell proliferation and for B cells producing immunoglobulins.

Strain Description


Strain   Name

C57BL/6-Cd27tm1(hCD27)/Bcgen

Background

C57BL/6

Related Genes

Cd27 (CD27   antigen)


Gene Description


CD27 is a TNF receptor super family member. It is expressed on the surface of T and B cells, and its  binding with CD70 provides a co-stimulatory signal for T and B cell proliferation and for immunoglobulin production by B cells. Treating mice with a CD27 agonist antibody enhances the anti-tumor immune response in the MC38 colon carcinoma model.


Targeting strategy


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Protein expression analysis


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Fig 1. Splenocytes from both wild type (WT) C57BL/6 and homozygous B-hCD27 mice were analyzed by flow cytometry.

Mouse CD27+ T cells were detected in both WT C57BL/6 and homozygous B-hCD27 mice, while human CD27+ T cells were only detected in the homozygous B-hCD27 mice. This might be due to cross-recognition of hCD27 by anti-mCD27 antibodies.


CD27 antibody efficacy evaluation


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Fig 2. Murine colon cancer MC38 cells were subcutaneously implanted into homozygote B-hCD27 mice. Mice were divided into control and treatment groups (n=5) when the tumor size was approximately 150±50 mm3. Anti-hCD27 antibody significantly inhibited tumor growth in  homozygote B-hCD27 mice, suggesting that the B-hCD27 mouse model is an effective tool for in vivo hCD27 antibody efficacy studies. (A) Tumor average volume ± SEM, (B) Mice average weight ± SEM.



 

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