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Animal Models and Cell Lines

>Single Humanized Immune-Checkpoint Mice

CD27 is a TNF receptor super family member. It is expressed on the surface of T and B cells, and its binding with CD70 provides a co-stimulatory signal for T and B cell proliferation and for B cells producing immunoglobulins.

Strain Description

Strain Name

C57BL/6-Cd27tm1(hCD27)/Bcgen

Common Name

B-hCD27 mice

Background

C57BL/6

Catalog number

B-EM-024

Catalog number (Males)

BEM024M

Catalog number (Females)

BEM024F

Related Genes

Cd27 (CD27 antigen)

Gene Description:

CD27 is a TNF receptor super family member. It is expressed on the surface of T and B cells, and its binding with CD70 provides a co-stimulatory signal for T and B cell proliferation and for B cells producing immunoglobulins. Treating mice with a CD27 agonist antibody effectively enhanced the antitumor immune response for lymphoma and B16 melanoma, providing putative targets for tumor immunotherapy. 

 

mRNA expression verification

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Fig 1. RT-PCR analysis of CD27 gene.

The hCD27, but not mCD27, mRNA was detected in splenocytes of the homozygous B-hCD27 mice.


Protein expression analysis


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Fig 2. Splenocytes from both wild type (WT) C57BL/6 and homozygous B-hCD27 mice were analyzed by flow cytometry.

Mouse CD27+ T cells were detected in both WT C57BL/6 and homozygous B-hCD27 mice, while human CD27+ T cells were only detected in the homozygous B-hCD27 mice. This might be due to cross-recognition of hCD27 by anti-mCD27 antibodies.


CD27 Ab efficacy evaluation

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Fig 3. Murine colon cancer MC38 cells were subcutaneously implanted into heterozygous B-hCD27 mice.

Mice were divided into control and treatment groups (n=5) when the tumor size was approximately 100 mm3. Anti-hCD27 antibody significantly inhibited tumor growth in the heterozygous B-hCD27 mice, suggesting that B-hCD27 mouse model is an effective tool for in vivo hCD27 antibody efficacy studies. The average±SEM of tumor sizes are shown in the figure.


 

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