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Animal Models and Cell Lines

>Single Humanized Immune-Checkpoint Mice

BTLA (B and T lymphocyte associated) is a B cell and T cell attenuator, another immune checkpoint negative regulator of the Ig superfamily.

Strain description

Strain Name

C57BL/6-Btlatm1(hBTLA)/Bcgen

Background

C57BL/6

Related Genes

Btla (B and T   lymphocyte associated)





Gene description

BTLA (B and T lymphocyte associated) is a B cell and T cell attenuator, another immune checkpoint negative regulator of the Ig superfamily. It is structurally similar to CTLA-4 and PD-1, and is expressed in B cells, T cells, NK cells, dendritic cells and macrophages. BTLA binds to its ligand HVEM (HVEM is a member of the tumor necrosis factor receptor superfamily) to transmit a co-inhibition signal in the body's anti-tumor immune response, and is associated with the immune escape mechanism of the tumor. BTLA inhibitors enhance the TCR signaling pathway, restore T cell function, and is a potential novel target for tumor biotherapy.


Targeting strategy


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Protein expression analysis

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Fig 1. Splenocytes from both wild type (WT) C57BL/6 and the B-hBTLA homozygous mice were analyzed by flow cytometry. 

Mouse BTLA+ B cells were detected in the WT mice, while human BTLA+ B cells were detected in the homozygous B-hBTLA  mice.



Human BTLA mAb efficacy evaluation (MC38-hHVEM cell line)


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Fig 2. Murine colon cancer MC38-hHVEM cells were subcutaneously implanted into homozygous B-hBTLA mice.

Mice were grouped when the tumor size was approximately 150±50mm3 (n=6). Two human BTLA antibodies inhibited tumor growth differently, confirming that the B-hBTLA mouse model is a powerful tool for in vivo BTLA antibody pharmacological efficacy studies. (A) Tumor average volume ± SEM, (B) Mice average weight ± SEM.

 

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