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>Single Humanized Immune-Checkpoint Mice

CD28 (Cluster of Differentiation 28) is one of the proteins expressed on T cells that provide co-stimulatory signals required for T cell activation and survival.

Strain description

Strain Name

 C57BL/6-Cd28tm1(hCD28)/Bcgen

Background

C57BL/6

Related Genes

Cd28 (CD28   antigen)


Gene Description

CD28 (Cluster of Differentiation 28) is one of the proteins expressed on T cells that provide co-stimulatory signals required for T cell activation and survival. T cell stimulation through CD28 in addition to the T-cell receptor (TCR) can provide a potent signal for the production of various interleukins (IL-2 and IL-6 in particular). CD28 is the only B7 receptor constitutively expressed on naive T cells. Association of the TCR of a naive T cell with MHC: antigen complex without CD28:B7 interaction results in a T cell that is anergic. Agonistic antibodies targeting CD28 have entered clinical trials.


Targeting strategy


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Protein expression analysis


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Fig 1. Splenocytes from both wild type (WT) C57BL/6 and heterozygous B-hCD28 mice were analyzed by flow cytometry. 

Mouse CD28+ T cells were detected in both WT C57BL/6 and heterozygous B-hCD28 mice, while human CD28+ T cells were only detected in the heterozygous B-hCD28 mice.


B-hCD28 model evalution

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Fig2. C57BL/6 and B-hCD28 mice were immuned by hPDL1 recombination protein with CFA . No significant difference was detected, indicating that the B7-1/CD28 co-stimulating signaling pathway was normal in hCD28 mice. Chimera of CD28 had efficient activity.  






 

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