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Animal Models and Cell Lines

>Single Humanized Immune-Checkpoint Mice

CD47 is widely expressed on cell surface and interacts with the signal regulatory protein α (SIRPα), thrombospondin-1 (TSP1) and integrins to mediate a series of responses including apoptosis, proliferation and immunity.

Strain description

Strain Name

BALB/c-Cd47tm1(hCD47)/Bcgen

Background

B-hCD47 mice(C)

Related Genes

Cd47 (CD47   antigen)


Gene Description

CD47 is an integrin associated protein (IAP) and is an immunoglobulin superfamily member. CD47 is widely expressed on cell surface and interacts with the signal regulatory protein α (SIRPα), thrombospondin-1 (TSP1) and integrins to mediate a series of responses including apoptosis, proliferation and immunity. CD47 is an important “self ” mark on the cell surface and inhibits macrophagocytosis by interaction with SIRPα on macrophage surface. Animal studies have shown that a CD47 antibody is an effective treatment for multiple types of tumors. CD47 is another target for tumor immunity following PD-1/PD-L1.


Mouse background

BALB/c : inbred strain used for oncology, immunology, neurobiology and inflammation studies, easily produces plasma cell tumors after injection of mineral oil. A variety of mouse tumor cell lines were derived from the BALB/c background, and this strain is good for tumor formation.


Targeting strategy


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Protein expression analysis


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Fig 2. Splenocytes from both wild type (WT) BALB/c and heterozygous B-hCD47 (B/c) mice were analyzed by flow cytometry. 

Mouse CD47+ T cells were detected in both WT BALB/c and heterozygous B-hCD47 (B/c) mice, while human CD47+ T cells were only detected in the heterozygous B-hCD47 (B/c) mice.




 

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