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Animal Models and Cell Lines

>Double Humanized Immune-Checkpoint Mice

B-hPD-1/hOX40 mice


Basic characteristics

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Targeting strategy


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Protein expression analysis

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Fig 1. Splenocytes from both wild type (WT) C57BL/6 and homozygous B-hPD-1/hOX40 mice were analyzed by flow cytometry. Mouse OX40+ T cells were detectable in the WT C57BL/6 mice, while human OX40+ T cells were detectable in the homozygous B-hPD-1/hOX40 mice.


 

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Fig 2. Splenocytes from both wild type (WT) C57BL/6 and homozygous B-hPD-1/hOX40 mice were analyzed by flow cytometry. Mouse PD-1+ T cells were detectable in the WT C57BL/6 mice, while human PD-1+ T cells were detectable in the homozygous B-hPD-1/hOX40 mice.

 

Combination therapy of PD-1 (Keytruda) mAb and OX40 mAb (MC38 cell line)

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Fig 3. Murine colon cancer MC38 cells were subcutaneously implanted into homozygous B-hPD-1/hOX40 mice. Mice were grouped when the tumor size was approximately 150±50 mm3 (n=6). Anti-hOX40 antibody and the anti-hPD-1 antibody Keytruda show inhibitory effects on tumor growth. On the other hand, combination of anti-hOX40 antibody and the anti-hPD-1 antibody Keytruda shows almost complete inhibition when compared to single Ab treatment. B-hPD-1/hOX40 mouse model is a powerful tool for is a powerful tool for in vivo efficacy evaluation of hPD-1 and hOX40 antibodies. (A) Tumor average volume ± SEM, (B) Mice average weight ± SEM.

 


Combination therapy of PD-1 (Keytruda) mAb and OX40 mAb (B16-F10-hPD-L1 cell line)

 

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Fig 4. Murine melanoma B16-F10-hPD-L1 cells were subcutaneously implanted into homozygous B-hPD-1/hOX40 mice. Mice were grouped when the tumor size was approximately 150±50 mm3 (n=5). Combination of anti-hOX40 antibody and the anti-hPD-1 antibody Keytruda shows higher inhibitory effects than single antibody treatment. B-hPD-1/hOX40 mouse model is a powerful tool for is a powerful tool for in vivo efficacy evaluation of hPD-1 and hOX40 antibodies combination therapy. (A) Tumor average volume ± SEM, (B) Mice average weight ± SEM..

 

 

 

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