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Animal Models and Cell Lines

>Double Humanized Immune-Checkpoint Mice

Basic characteristics

 

Strain NameC57BL/6-Pdcd1tm1(hPDCD1)Cd137tm1(hCD137)/BcgenCommon NameB-hPD-1/h4-1BB mice
BackgroundC57BL/6Catalog numberB-DM-001
Catalog number (Males)BDM001MCatalog number (Females)BDM001F
Related Genes

PD-1(Programmed death-1) ;

TNFRSF9(TNF receptor superfamily member 9, also known as 4-1BB

 

Targeting strategy


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Protein expression analysis 

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Fig 1. Splenocytes from both wild type (WT) C57BL/6 and homozygous B-hPD-1/h4-1BB mice were analyzed by flow cytometry.

Mouse 4-1BB+ T cells were detected in the WT C57BL/6 mice, while human 4-1BB+ T cells were detected in the homozygous B-hPD-1/h4-1BB mice.


 

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Fig 2. Splenocytes from both wild type (WT) C57BL/6 and homozygous B-hPD-1/h4-1BB mice were analyzed by flow cytometry.

Mouse PD-1+ T cells were detected in the WT C57BL/6 mice, while human PD-1+ T cells were detected in the homozygous B-hPD-1/h4-1BB mice.

 

Combination therapy of PD-1 (Keytruda) mAb and 4-1BB antibody (Urelumab Analog) (MC38 cell line)

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Fig 3. Murine colon cancer MC38 cells were subcutaneously implanted into homozygous B-hPD-1/h4-1BB mice.

Mice were grouped (n=5) when the tumor size was approximately 150 ± 50mm3 (n=6). Anti-hPD-1 antibody and anti-h4-1BB antibody significantly inhibited tumor growth in the homozygous B-hPD-1/h4-1BB mice, suggesting that B-hPD-1/h4-1BB mouse model is an effective tool for in vivo efficacy evaluation of hPD-1 and h4-1BB antibody combination therapy. (A) Tumor average volume ± SEM, (B) Mice average weight ± SEM.



 

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