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Animal Models and Cell Lines

>Double Humanized Immune-Checkpoint Mice

Basic characteristics

Strain Name C57BL/6-Pdcd1tm1(hPDCD1)Cd27tm1(hCD27)/Bcgen  Common NameB-hPD-1/hCD27 mice
Background  C57BL/6Catalog number B-DM-003
Catalog number (Males) BDM003M Catalog number (Females) BDM003F

Related Genes

PD-1(Programmed death-1) ;

CD27(CD27 molecule)


Targeting strategy

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Protein expression analysis

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Fig 1. Splenocytes from both wild type (WT) C57BL/6 and homozygous B-hPD-1/hCD27 mice were analyzed by flow cytometry. 

Mouse CD27+ T cells were detected in both WT  C57BL/6 and the homozygous B-hPD-1/hCD27 mice, while human CD27+ T cells were detected in the homozygous B-hPD-1/hCD27 mice. This might result from the crossrecognition of hCD27 by anti-mCD27 antibodies.


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Fig 2. Splenocytes from both wild type (WT) C57BL/6 and homozygous B-hPD-1/hCD27 mice were analyzed by flow cytometry. 

Mouse PD-1+ T cells were detected in the WT C57BL/6 mice, while human PD-1+ T cells were detected in the homozygous B-hPD-1/hCD27 mice.



 

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