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>The mouse model and efficacy evaluation of immunological checkpoint

Gene description

CD27 is a TNF receptor super family member. It is expressed on the surface of T and B cells, and its  binding with CD70 provides a co-stimulatory signal for T and B cell proliferation and for B cells producing immunoglobulins. Treating mice with a CD27 agonist antibody effectively enhanced the antitumor immune response for lymphoma and B16  melanoma, providing putative targets for tumor immunotherapy.

mRNA expression analysis


Fig 1. RT-PCR analysis of CD27 gene.

ThehCD27, but not mCD27, mRNA was detected in splenocytes of the homozygous B-hCD27 mice.

Protein expression analysis


Fig 2. Splenocytes from both wild type (WT) C57BL/6 and homozygous B-hCD27 mice were analyzed by flow cytometry. Mouse CD27+ T cells were detected in both WT C57BL/6 and homozygous B-hCD27 mice, while human CD27+ T cells were only detected in homozygous B-hCD27 mice. This might be due to cross-recognition of hCD27 by anti-mCD27 antibodies.

Human CD27 mAb efficacy evaluation (MC38 cell line)


Fig 3. Murinecolon cancer MC38 cells were subcutaneously implanted into heterozygous B-hCD27 mice. Mice were divided into control and treatment groups (n=5) when the tumor size was approximately 100 mm3. Anti-hCD27 antibody significantly inhibited tumor growth in heterozygous B-hCD27 mice, suggesting that the B-hCD27 mouse model is an effective tool for in vivo hCD27 antibody efficacy studies. (A) Tumor average volume ± SEM, (B) Mice average weight ± SEM.

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