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Gene Targeting Services

>ESC Based Gene Gene KO/KI Targeting


A conditional Knockout (cKO) is usually generated through the Cre-LoxP/Flp-Frtrecombination systems. The candidate fragment that will be knocked out is flanked by LoxP or Frtelements.

A conditional Knockout (cKO) is usually generated through the Cre-LoxP/Flp-Frtrecombination systems. The candidate fragment that will be knocked out is flanked by LoxP or Frtelements. The floxed(flanked by LoxP or Frt) mice are bred with tissue-specific Cre (or Flp) mice, so that the flanked sequence will be removed  from the offsprings’ genome in a tissue-specific pattern. For the conditional knockout generation, theloxPfragments are usually inserted into the introns downstream of the ATG-containingexon, and removal of the flanked exon (s) will result in a frame-shift that disrupts protein expression.

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