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Gene Targeting Services

>ESC Based Gene Gene KO/KI Targeting



The traditional transgenic mouse models are usually constructed via pronuclear injection of plasmid, and many different founders can be obtained.

The traditional transgenic mouse models are usually constructed via pronuclear injection of plasmid, and many different founders can be obtained. The experimentalresults from different founder mice can be quite different and not reproducible due to the difference in integration copy number and loci. Currently, most researchers use site-specific integration strategies to build transgenic mouse models. Rosa26 is the most commonly used locus because Rosa26 encodes a nonessential nuclear RNA expressed in almost all tissues. The LoxP-3X-STOP-LoxPin front of the knockin gene can achieve the conditional expression of the exogenous gene  through crossing with suitable Cre  mouse lines.

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