Gene Targeting Strategy
The Col2a1 gene encodes the alpha-1 chain of type II Collagen, a fibrillar collagen found in cartilage and the vitreous humor of the eye. In this strain, Cre recombinase expression is under the control of Col2a1 promoter in a chondrocyte-specific pattern. When crossed with a strain containing a loxP site-flanked sequence of interest, Cre-mediated recombination results in deletion of the flanked sequence in Col2a1 expressing cells.
Mice hemizygous for Col2a1iCre gene are viable and fertile without any physical abnormalities.
(B-EM-002) B-Col2a1-iCre mice