Southern blot – “Gold Standard” for mouse knock-out quality control
Biocytogen’s Extreme Genome Editing System (EGE) is a set of high efficiency gene knockout/knockin methods based on CRISPR/Cas9 technology and developed and optimized by Biocytogen (hereinafter referred to as the EGE system).
Three essential factors for mouse breeding
EGE can be widely used for high-efficiency genome editing of various eukaryotic systems, including gene fixed-point knockouts, point mutations, knockin, etc. It can be used in rats, mice and various cell lines. Biocytogen has successfully prepared more than 200 types of knockouts, conditional knockout and knockin mouse models, more than 50 types of rat models and more than 70 types of fixed-point gene editing cell lines (human embryonic stem cells/ips cells and multiple types of cancer cell lines, etc.) by making use of EGE.
What are the advantages of EGE technology compared to CRISPR/Cas9 technology?
The use of CRISPR/Cas9 technology for gene spot mutation and knockin is based on homologous recombination of the genome; the homologous recombination efficiency of exogenous DNA mediated by common CRISPR/Cas9 technology is low. Through a series of innovation and optimization strategies, the homologous recombination efficiency for the EGE system developed by Biocytogen is increased by 10-20 times when compared to traditional CRISPR/Cas9 technology, making gene modification with EGE more efficient, faster, and convenient.
Why are mice the ideal animal model for basic biological research?
Customers only need to provide the name of gene to be used and any special requirements. We provide consulting services for free for inexperienced customers, in order to clearly define your needs and requirements and provide you the optimal design scheme. We also perform a sequence analysis for the gene, and then give you a preliminary design. After constant communication between us and the customer, and when consensus is reached between both parties, a technical service agreement is signed. We will then prepare your model until you ultimately receive your desired F1 mouse.
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