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B-NDG mice (Catalog number:B-CM-002)

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B-NDG mice

B:Biocytogen;  N:NOD background;  D:DNAPK(Prkdc)null;G:IL2rg knockout.

NOD-Prkdcscid IL2rgtm1/Bcgen

B-NDG mice are mice with NOD genetic  background and Prkdc and IL2rg double gene knockout. They are  internationally accepted mouse modl with the highest degree of  immunodeficiency, and they are suitable for human cells or tissue  transplantation.


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Basic features:

NOD (non-obese diabetes) genetic background: spontaneous type-I diabetes; macrophages have weak  human cell cytophagy; innate immune system functions such as complement system and dendritic cells are reduced.

Prkdc(scid): Prkdc (protein kinase DNA-activated catalytic) gene mutation;  functional T cells and B cells of mice are lost; lymphocytes are reduced; manifested to have severe combined immune deficiency (scid) of cellular immunity and humoral immunity.

IL2rgnull: the gamma chain of Interleukin-2 receptor (IL-2R γc, also called as CD132) is on the mouse X chromosome, and is the common receptor subunit of cytokines IL2, IL-4, IL-7, IL-9, IL-15 and IL-21 with significant immune functions; after the gene is knocked out, mouse immunity function is greatly weakened, in particular activities of NK cells, which are nearly lost.

B-NDG mice: comprehensive background features of NOD-SCID-IL2rg, with severe immunodeficiency phenotype; no mature T cells, B cells or functional NK cells; deficient in cytokine signaling capacity.  Very suitable for transplantation and growth of human hematopoietic stem cells and peripheral blood mononuclear cells.



MeritsMain application
Mouse model with currently the highest immunodeficiency degree in the worldHuman-derived cells or tissues transplantation
Longer lifespan than that of NOD-scid mice; 1.5 years on averageTumor and tumor stem cell study
Almost no rejection of human-derived cells and tissuesES and iPS cell study
Some cells can form tumors, depending on cell lines or cell typesHematopoiesis and immunology study
No B lymphocyte leakageHuman disease infection model study

New humanized animal model study



Preliminary phenotypic analysis


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Fig.1 Complete loss of T, B and NK cells of B-NDG mice. Spleen cells of C57BL/6, BALB/c, NOD-scid and B-NDG mice were isolated, and compositions of T, B and NK cells were characterized using flow cytometry and statistically compared.


图片2.pngFig.2 Successful engraftment of hPBMCs (human peripheral blood mononuclear cells) in B-NDG mice.      Twenty-four days after injecting  5X106 of human peripheral blood mononuclear cells (hPBMCs) into the tail vein of B-NDG mice, blood was isolated and subjected to FACS analysis to detect ratio of hCD45+ to mCD45+. Similar results were obtained for all three B-NDG mice.



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Fig.3 Successful engraftment of Raji cells in B-NDG mice The same amount (5X106) of Raji cells were injected into B-NDG, NOD-scid and BALB/c Nude mice. We recorded and analyzed the following indicators  in the mice at different time points.
A. Record the living situation of mice after cell inoculation, and construct Kaplan-Merier survival curves.
B. Measure the weight (g) change each week after inoculation, and calculate rweight relative to weight at day of inoculation.
C. Measure the percentage change of human cells in mouse peripheral blood. After inoculation of Raji cells, take 100μl whole blood via retro-orbital venous plexus each week, extract DNA, and detect ratio of human cells in peripheral blood of mice by q-PCR.
D. Compare livers of mice after Raji cell inoculation. After the weight of mice reduces by more than 20% after inoculation, perform euthanasia, dissect the viscera, and take photos.
E. Immunohistochemical staining of mice viscera after Raji cell inoculation. After the weight of mice reduces by more than 20% after inoculation, perform euthanasia, dissect the mice, and embed livers and spleens into wax for immunohistochemical assay. The primary antibody is murine anti-human mitochondrial membrane protein antibody (Millipore, MAB1273), and the magnification is 400x

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Fig.4: B-NDG is a better choice for modeling in comparison to CB17-scid mice,
(Since gastric cancer samples of patients are generally modeled with CB17-scid mice at present, CB17-scid mice are taken as control in this test.)
A. Gastric cancer samples of patients were inoculated subcutaneously into B-NDG mice, which formed tumors.
B. Tumor growth curve of gastric cancer samples of patients in B-NDG mice bodies.

C. Comparison of tumor take rate of gastric cancer samples in CB17-scid mice and B-NDG mice bodies.


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