Results
Orthotopic Melanoma Model: B-Tg(CAG-Luc-EGFP) A375+ B-NDG mice (orthotopic)
| Study ID: |
Groups |
Animal strain |
Sex |
Cell line |
The amount of
cells Inoculated |
The Volume
of cells |
Animal number |
Remarks |
| 24P071547 |
G1 |
B-NDG mice |
F |
B-Tg(CAG-Luc-EGFP) A375#1-B08 |
5×104 |
0.05ml/mice |
6 |
i.d.injection |
| G2 |
B-NDG mice |
F |
B-Tg(CAG-Luc-EGFP) A375#1-B08 |
1×106 |
0.05ml/mice |
6 |
i.d.injection |
| G3 |
B-NDG mice |
F |
B-Tg(CAG-Luc-EGFP) A375#1-B08 |
1×106 |
0.2ml/mice |
6 |
i.v injection |
Establishment of orthotopic melanoma model.
The B-NDG mice (F, n=6) were inoculated with B-Tg(CAG-Luc-EGFP) A375 cell suspension (5×10⁴, 1×10⁵, 1×10⁶) intradermally or intravenously. And the body weight and tumor fluorescence signal of the mice were recorded weekly. The results showed that the fluorescence signal gradually increased. This indicates that the cell line was successfully constructed as an orthotopic tumor model.
Orthotopic Epidermoid Carcinoma Model: B-CMV-Luc A-431+ B-NDG mice (orthotopic)
| Study ID: |
Groups |
Animal strain |
Sex |
Cell line |
The amount of
cells Inoculated |
The Volume
of cells |
Animal number |
Remarks |
| 24P071548 |
G1 |
B-NDG mice |
F |
B-CMV-Luc A-431#2-H01 |
5×104 |
0.05ml/mice |
6 |
i.d.injection |
| G2 |
B-NDG mice |
F |
B-CMV-Luc A-431#2-H01 |
1×105 |
0.05ml/mice |
6 |
i.d.injection |
| G3 |
B-NDG mice |
F |
B-CMV-Luc A-431#2-H01 |
1×106 |
0.2ml/mice |
6 |
i.v injection |
Establishment of orthotopic epidermoid carcinoma model.
The B-NDG mice (F, n=6) were inoculated with B-CMV-Luc A-431 cell suspension (5×10⁴, 1×10⁵, 1×10⁶) intradermally or intravenously. And the body weight and tumor fluorescence signal of the mice were recorded weekly. The results showed that the fluorescence signal gradually increased. This indicates that the cell line was successfully constructed as an orthotopic tumor model.
