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Reporter/tag mouse model

1.Definition

A mouse model where a reporter gene (such as EGFP, mRFP, mCherry, mYFP or LacZ, etc.) or a tagged gene is introduced into the target gene position. The transcriptional activity of the target gene promoter, positioning, and cell transfer is monitored by reporter gene expression.

2.Advantages and disadvantages

Advantages: the reporter/tag mouse model is a useful tool for in vivo study of the promoter's transcriptional activity,  or the protein's positioning or cell transfer.

Disadvantages: The target gene would be knocked out if the reporter gene is introduced downstream of the target gene's promoter and upstream of its coding sequence. IRES or 2A technology can be used to avoid the target gene knockout, and the target gene and reporter gene can be co-expressed.

3. Principle

If the reporter/tag mouse model is used to monitor the promoter's transcriptional activity , the target gene's coding sequence can be replaced by the coding sequence of reporter gene (EGFP, for example), and the target gene will be knocked out when the reporter gene is introduced.

If the reporter/tag mouse model is used to monitor the expression, positioning, and cell transfer of the protein, the protein can be tagged by adding a short peptide coding sequence at the end of the target gene's protein-coding region. The target protein's expression and functions can be indirectly studied by identifying the short peptide with the short peptide antibody. This method is particularly suitable for a target protein that lacks a suitable antibody.

4.Application

Study of the gene promoter's transcriptional activity

Monitor the expression, positioning, and cell transfer of the protein in vivo

Drug target affirmation

Preclinical drug evaluation


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