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B-hPD-L1 mice (PD-L1 humanized mice)

2017-05-09 17:42:00.0

Basic characteristics:


PD-L1 (programmed cell death ligand-1), also known as B7-H1 and CD274, is mainly expressed in antigen-presenting cells (APCs) and activated T cells. The interaction between PD-L1 and its ligand, PD-1, plays a significant role in the negative regulation of the immune response. PD-L1 is highly expressed in a variety of solid tumors. PD1 and PD-L1 interactions reduce T cell activation and promote tumor immune escape. Anti-tumor immune response can be restored by using anti-PD-1 or anti-PD-L1 antibodies to block the interactions between PD-1 to PD-L1.


Targeting strategy:



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mRNA expression verification:

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Fig 1. Molecular identification of the B-hPD-L1 humanized mice. Mouse PD-L1 mRNA was detected in both the wild type and the B-hPD-L1 heterozygous mice, while human PD-L1 mRNA was detected only in the B-hPD-L1 heterozygous mice.


Protein expression verification:

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Fig 2. Abdominal lymph cells from both wild type (WT) C57BL/6 and the B-hPD-1 homozygous mice were analyzed by flow cytometry. Mouse PD-L1+ B cells were detected in the WT mice, while human PD-L1+ B cells were detected in the B-hPD-1 homozygous mice.



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Fig 3. Spleen cells from both wild type (WT) C57BL/6 and B-hPD-1 homozygous mice were analyzed by flow cytometry. Mouse PD-L1+ T cells were detected in the WT mice, while human PD-L1+ T cells were detected in the B-hPD-1 homozygous mice.



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