中文 | English     

Prepare the animal model by TALEN technology

1. Brief introduction of the technology

TALEN (Transcription Activator-Like Effector Nuclease) is another high-efficiency genome modification tool. Its high efficiency, strong specificity, low missing rate, and simple design makes the technology widely used for gene knockout and genome modification at the cell and in vivo levels.

2. Preparation flow

1464249825797068942.png 

3. Service process

BIOCYTOGEN provides services for mouse model construction in one step, including model designing, construction, and genotypic identification service

 

3.1 Project feasibility evaluation and model design

BIOCYTOGEN possesses a senior technical team specializing in comprehensive analysis of project feasibility, and provides a free and professional model design service. The services include:

Ÿ Define the purpose for constructing the model

Ÿ Analyze the target gene’s structure and sequence information

Ÿ Analyze existing data

Ÿ Design all possible model construction schemes

Ÿ Analyze the advantages and risks of all model construction schemes

Ÿ Accurate prediction of project implementation time and quotation

 

3.2 Design and construction of TALEN

3.3 Pronuclear injection

Ÿ Pronuclear injection of the mRNA (TALEN) into the mouse zygotes

3.4 Obtain the mouse of generation F0 and breeding

Ÿ Recipient female mouse feeding and the birth of chimeric mouse

Ÿ Mating of chimeric mouse and wild mouse

3.5 Obtain the phyla-genetic hybrid mouse of generation F1

Ÿ Birth of generation F1 mice

Ÿ Ensure the phyla heredity by identification of generation F1 mouse tail genotype

 

4. Advantages of the TALEN service


All-round technology optimization

High gene knockout efficiency

Accurate TALEN knockout activity evaluation

RVD specificity identification

The TALEN plasmid with correct sequencing can be obtained in five days

Directly evaluate the activity in the target cell line, which mirrors the environmental level of zygotes

Spacer length

The activity evaluation in the cell line can be finished in 1 to 2 weeks

Construction of TALEN module

The rat with a whole-gene knockout can be obtained in 4 to 6 months

Establishment of TALEN module resource base









Copyright © BIOCYTOGEN LLC. 2017 All Rights Reserved.