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B-hCD274(PD-L1) mice

2017-05-09 17:42:00.0

Strain description


Strain NameC57BL/6-Cd274tm1(hCD274)/BcgenCommon NameB-hCD274(PD-L1) mice
BackgroundC57BL/6Catalog numberB-CM-029
Catalog number (Males)BCM029MCatalog number (Females)BCM029F
Related GenesCd274 (CD274 antigen)

Gene description

PD-L1 (programmed cell death ligand-1), also known as B7-H1 and CD274, is mainly expressed in antigen-presenting cells (APCs) and activated T cells. The interaction between PD-L1 and its ligand, PD-1, plays a significant role in the negative regulation of the immune response. PD-L1 is highly expressed in a variety of solid tumors. PD1 and PD-L1 interactions reduce T cell activation and promote tumor immune escape. Anti-tumor immune response can be restored by using anti-PD-1 or anti-PD-L1 antibodies to block the interactions between PD-1 to PD-L1.


Targeting strategy



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mRNA expression analysis

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Fig 1.RT-PCR analysis of PD-L1 gene.

The hPD-L1, but not mPD-L1, m RNA was detected in splenocy tes of the h omoz ygous B -hPD-L1 mice.



Protein expression analysis

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Fig 2. Abdominal lymph cells from both wild type (WT) C57BL/6 and the B-hPD-1 homozygous mice were analyzed by flow cytometry. Mouse PD-L1+ B cells were detected in the WT mice, while human PD-L1+ B cells were detected in the homozygous B-hPD-1  mice.







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Fig 3. Spleen cells from both wild type (WT) C57BL/6 and B-hPD-L1 homozygous mice were analyzed by flow cytometry. 

Mouse PD-L1+ T cells were detected in the WT mice, while human PD-L1+ T cells were detected in the homozygous B-hPD-1  mice.







PD-L1(Tecentriq) Ab effic acy e valuation (different doses)

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Fig 4. Modified murine colon cancer MC38 cells (mPD-L1 extracellular domain is replaced with the hPD-L1 extracellular domain,named as MC38-hPD-L1) were subcutaneously implanted into homozygous B-hPD-L1 mouse. Mice were divided into control and experimental groups (n=5) when the tumor size was about 100 mm3. The anti-hPD-L1 antibody Tencentiq (Atezolizumab ) significantly inhibited tumor growth in the homozygous B-hPD-L1 mice at 3 different doses, suggesting that MC38-hPD-L1 cells in B-hPD-L1 mouse model is an effective tool for in vivo hPD-L1 antibody efficacy studies. (A) Tumor average volume ± SEM, (B) Mice average weight ± SEM.





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