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B-hTNFRSF4(OX40) mice

2017-05-09 17:41:18.0

Strain description


Strain NameC57BL/6-Tnfrsf4tm1(hTnfrsf4)/BcgenCommon NameB-hTNFRSF4(OX40) mice
BackgroundC57BL/6Catalog numberB-CM-027
Catalog number (Males)BCM027MCatalog number (Females)BCM027F
Related GenesTnfrsf4 (tumor necrosis factor receptor superfamily, member 4)


Gene description

OX40, also known as Tnfrsf4 (Tumor necrosis factor receptor super family, member 4), is mainly expressed on the surface of activated CD4+ and CD8+ T cells, and its binding with the OX40 ligand can stimulate CD8+ T cell activation. The coactivation of OX40/OX40L enhances T cell function, including cytokine production, proliferation and T cell survival. An OX40 agonist can reduce Regulatory T cells (Tregs) and improve anti-tumor activity.


Targeting strategy


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mRNA expression verification

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Fig 1. RT-PCR analysis of OX40 gene.

The hOX40, but not mOX40, mRNA was detected in splenocytes of the homozygous B-hOX40 mice.



Protein expression analysis

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Fig 2. Spleen cells from both wild type (WT) C57BL/6 and the B-hOX40 homozygous mice were analyzed by flow cytometry. 

Mouse OX40+ T cells were detected in the WT mice, while human OX40+ T cells were detected in the homozygous B-hOX40  mice.







OX40 Ab efficacy evaluation (different doses)

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Fig 3. Murine colon cancer cells (MC38) were subcutaneously implanted into heterozygous B-hOX40 mice. Mice were divided into control and experimental groups (n=5) when the tumor size was about 100 mm3. Anti-hOX40 antibody significantly inhibited tumor growth in the heterozygous B-hOX40 mice at 3 different doses, suggesting that the B-hOX40 mouse model is an effective tool for in vivo hOX40 antibody efficacy studies. (A) Tumor average volume ± SEM, (B) Mice average weight ± SEM.

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Fig 4. Murine colon cancer cells (MC38) were subcutaneously implanted into homozygous B-hOX40 mice. Mice were divided into control and experimental groups (n=5) when the tumor size was about 100 mm3. The Anti-hOX40 antibody significantly inhibited tumor growth in the homozygous B-hOX40 mice, suggesting that the B-hOX40 mouse model is an effective tool for in vivo hOX40 antibody efficacy studies. (A) Tumor average volume ± SEM, (B) Mice average weight ± SEM.





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