|Strain Name||C57BL/6-Tnfrsf9tm1(hTNFRSF9)/Bcgen||Common Name||B-hTNFRSF9(4-1BB) mice|
|Catalog number (Males)||BEM023M||Catalog number (Females)||BEM023F|
|Related Genes||Tnfrsf9 (tumor necrosis factor receptor superfamily, member 9)|
TNFRSF9 (TNF receptor super family member 9), also called CD137 or 4-1BB, is mainly expressed on the surface of activated T, NK,and mononuclear cells. CD137 is activated by its ligand CD137L or an activating CD137 monoclonal antibody, and stimulates T cell activation, proliferation and cytokine production. This co-stimulatory signal can also multiply antigen presenting cells and result in cell factor secretion. Experiments show that CD137 co-stimulation regulates T cell and antigen presenting cell function for antitumor immunity, providing new targets for tumor immunological therapies.
mRNA expression verification
Fig 1. RT-PCR analysis of 4-1BB gene.
The h4-1BB, but not m4-1BB, mRNA was detected in splenocytes of the homozygous B-h4-1BB mice.
Protein expression analysis
Fig 2. Spleen cells from both wild type (WT) C57BL/6 and the B-h4-1BB homozygous mice were analyzed by flow cytometry.
Mouse 4-1BB+ T cells were detected in the WT mice, while human 4-1BB+ T cells were detected in the homozygous B-h4-1BB mice.
4-1BB Abs efficacy evaluation
Fig 3. Murine colon cancer MC38 cells were subcutaneously implanted into heterozygous B-h4-1BB mice. Mice were divided into control and treatment groups (n=5), which included 2 antibodies at 2 doses, tested when the tumor size was approximately 100 mm3. Anti-h4-1BB antibody Ab2 showed significant inhibitory effects at both doses, while Ab1 only inhibited tumor growth at the higher dose for heterozygous B-h4-1BB mice, suggesting that B-h4-1BB mouse model is a useful tool for in vivo h4-1BB antibody efficacy studies. (A) Tumor average volume ± SEM, (B) Mice average weight ± SEM.
Fig 4. Murine colon cancer MC38 cells were subcutaneously implanted into homozygous B-h4-1BB mice. Mice were divided into control and treatment groups (n=6), when the tumor size was approximately 150±50 mm3. Anti-h4-1BB antibody showed significant inhibitory effects in homozygous B-h4-1BB mice, suggesting that B-h4-1BB mouse model is a useful tool for in vivo h4-1BB antibody efficacy studies. (A) Tumor average volume ± SEM, (B) Mice average weight ± SEM.