中文 | English     

B-hCD137(4-1BB) mice

2017-05-09 17:41:08.0

Strain description


Strain NameC57BL/6-Tnfrsf9tm1(hTNFRSF9)/BcgenCommon NameB-hTNFRSF9(4-1BB) mice
BackgroundC57BL/6Catalog numberB-EM-023
Catalog number (Males)BEM023MCatalog number (Females)BEM023F
Related GenesTnfrsf9 (tumor necrosis factor receptor superfamily, member 9)


Gene description

TNFRSF9 (TNF receptor super family member 9), also called CD137 or 4-1BB, is mainly expressed on the surface of activated T, NK,and mononuclear cells. CD137 is activated by its ligand CD137L or an activating CD137 monoclonal antibody, and stimulates T cell activation, proliferation and cytokine production. This co-stimulatory signal can also multiply antigen presenting cells and result in cell factor secretion. Experiments show that CD137 co-stimulation regulates T cell and antigen presenting cell function for antitumor immunity, providing new targets for tumor immunological therapies.


Targeting strategy


图片.png

mRNA expression verification

图1.png


Fig 1. RT-PCR analysis of 4-1BB gene.

The h4-1BB, but not m4-1BB, mRNA was detected in splenocytes of the homozygous B-h4-1BB mice.



Protein expression analysis

图2.png




Fig 2. Spleen cells from both wild type (WT) C57BL/6 and the B-h4-1BB homozygous mice were analyzed by flow cytometry. 

Mouse 4-1BB+ T cells were detected in the WT mice, while human 4-1BB+ T cells were detected in the homozygous B-h4-1BB  mice.








4-1BB Abs efficacy evaluation

图3.png

Fig 3. Murine colon cancer MC38 cells were subcutaneously implanted into heterozygous B-h4-1BB mice. Mice were divided into control and treatment groups (n=5), which included 2 antibodies at 2 doses, tested when the tumor size was approximately 100 mm3. Anti-h4-1BB antibody Ab2 showed significant inhibitory effects at both doses, while Ab1 only inhibited tumor growth at the higher dose for heterozygous B-h4-1BB mice, suggesting that B-h4-1BB mouse model is a useful tool for in vivo h4-1BB antibody efficacy studies. (A) Tumor average volume ± SEM, (B) Mice average weight ± SEM.

图4.png

Fig 4. Murine colon cancer MC38 cells were subcutaneously implanted into homozygous B-h4-1BB mice. Mice were divided into control and treatment groups (n=6), when the tumor size was approximately 150±50 mm3. Anti-h4-1BB antibody showed significant inhibitory effects in homozygous B-h4-1BB mice, suggesting that B-h4-1BB mouse model is a useful tool for in vivo h4-1BB antibody efficacy studies. (A) Tumor average volume ± SEM, (B) Mice average weight ± SEM.


Copyright © BIOCYTOGEN LLC. 2017 All Rights Reserved.