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B-hCD47 mice (CD47 humanized mice)

2017-05-09 17:40:16.0

Basic characteristics:


CD47 is an integrin associated protein (IAP) and a member of the immunoglobulin superfamily. CD47 interacts with the signal regulatory protein α (SIRPα), thrombospondin-1 (TSP1) and integrins to mediate a series of responses including apoptosis, multiplication and immunity. CD47 is an important “self” mark on the cell surface by activating its ligand SIRPα on the macrophage surface to suppress macrophagecytophagy. Animal tests have shown that a CD47 antibody is an effective treatment for multiple types of tumors. CD47 antibody is considered as the target spot of next generation of tumor immunity suppressor following PD-1/PD-L1 antibody treatment.

 

Targeting strategy:


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mRNA expression verification:

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Fig 1. Molecular identification of the B-hCD47 humanized mice. Mouse CD47 mRNA was detected in both the wild type and the B-hCD47 heterozygous mice, while human CD47 mRNA was detected only in the B-hCD47 heterozygous mice.


Protein expression verification:

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Fig 2. Spleen cells from both wild type (WT) C57BL/6 and the B-hCD47 homozygous mice were analyzed by flow cytometry. Mouse CD47+ T cells were detected in the WT mice, while human CD47+ T cells were detected in the B-hCD47 homozygous mice.

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