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B-hCD47 mice

2017-05-09 17:40:16.0

Strain description


Strain NameC57BL/6-Cd47tm1(hCD47)/BcgenCommon NameB-hCD47 mice
BackgroundC57BL/6Catalog numberB-CM-021
Catalog number (Males)BCM021MCatalog number (Females)BCM021F
Related GenesCd47 (CD47 antigen)

Gene description

CD47 is an integrin associated protein (IAP) and is an immunoglobulin superfamily member. CD47 is widely expressed on cell surface and interacts with the signal regulatory protein α (SIRPα), thrombospondin-1 (TSP1) and integrins to mediate a series of responses including apoptosis, proliferation and immunity. CD47 is an important "self" mark on the cell surface and inhibits macrophagocytosis by interaction with SIRPα on macrophage surface. Animal studies have shown that a CD47 antibody is an effective treatment for multiple t ypes of tumors. CD47 is another target for tumor immunity following PD-1/PD-L1.

 

Targeting strategy


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mRNA expression analysis

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Fig 1. RT-PCR analysis of CD47 gene.

The hCD47, but not mCD47, mRNA was detected in splenocytes of the homozygous B-hCD47 mice.



Protein expression analysis

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Fig 2. Splenocytes from both wild type (WT) C57BL/6 and the B-hCD47 homozygous mice were analyzed by flow cytometry. 

Mouse CD47+ T cells were detected in the WT mice, while human CD47+ T cells were detected in the B-hCD47 homozygous mice.







CD47 Abs toxicity evaluation

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Fig 3. Homozygous B-hCD47 mice were injected with various antihCD47 antibodies via i.p. injection. Body weight was measured daily. The results indicated toxicity of different antibodies. hCD47 Ab4 and hCD47 Ab6 showed high toxicity levels, as mice died. Y-axis showed average body weight ± SEM.

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Fig 4. Homozygous B-hCD47 mice were i.p. injected with anti-hCD47 antibodies. Blood from each mice were collected and analyzed by Blood Routine Test. Blood from Ab3 and Ab5 groups were not analyzed because of death of the treated mice.

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Fig 5. Homozygous B-hCD47 mice were IP injected with anti-hCD47 antibodies. Blood from each mice were collected and analyzed by Blood Biochemical Test. Blood from Ab3 and Ab5 groups were not analyzed because of death of the treated mice.


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