中文 | English     

B-hTIGIT mice

2017-05-09 17:40:03.0

Strain description


Strain NameC57BL/6-Tigittm1(hTIGIT)/BcgenCommon NameB-hTIGIT mice
BackgroundC57BL/6Catalog numberB-CM-031
Catalog number (Males)BCM031MCatalog number (Females)BCM031F
Related GenesTigit (T cell immunoreceptor with Ig and ITIM domains)


Gene description

TIGIT (T-cell immunoreceptor with Ig and ITIM domains), a member of the immunoglobulin family of PVR (poliovirus receptor), is a type I transmembrane protein. Similar to PD-1, TIM3, and L AG3, TIGIT is highly expressed in cancer tissues and competitively binds to TIGIT receptors with DNAM-1 proteins on the surface of NK cells to reduce the NK cell killing efficiency of cancer cells. Therefore, anti-TIGIT antibody can enhance NK cell ability to destroy tumor cells by facilitating DNAM-1 binding to TIGIT receptors. It has become the new generation of cancer immunotherapy checkpoint.


Targeting strategy

10 B-hTIGIT 小鼠.png

 


mRNA expression analysis

图1.png


Fig 1. RT-PCR analysis of TIGIT gene.

The hTIGIT, but not mTIGIT, mRNA was detected in splenocytes of the homozygous B-hTIGIT mice.




Protein expression analysis

图2.png





Fig 2. Splenocytes from both wild type (WT) C57BL/6 and homozygous B-hTIGIT mice were analyzed by flow cytometry.

Mouse TIGIT+ T cells were detected in the WT mice, while human TIGIT+ T cells were detected in the homozygous B-hTIGIT mice.






TIGIT Ab efficacy  evaluation

图3.png

Fig 3. Murine colon cancer MC38 cells were subcutaneously implanted into homozygous B-hTIGIT mice. Mice were divided into control and treatment groups (n = 5) when the tumor volume was about 100 mm3. The anti-human TIGIT antibody inhibits tumor growth in the homozygous B-hTIGIT mice, suggesting that the B-hTIGIT mouse model is an effective tool for in vivo hTIGIT antibody efficacy study. (A) Tumor average volume ± SEM, (B) Mice average weight ± SEM.



Copyright © BIOCYTOGEN LLC. 2017 All Rights Reserved.