|Strain Name||C57BL/6-Havcr2tm1(hHAVCR2)/Bcgen||Common Name||B-hHAVCR2(TIM3) mice|
|Catalog number (Males)||BCM032M||Catalog number (Females)||BCM032F|
|Related Genes||Havcr2 (hepatitis A virus cellular receptor 2)|
TIM3 (T-cell immunoglobulin domain and mucin domain-3) is an activation-induced inhibitory molecule involved in immune tolerance and T-cell exhaustion in chronic viral infection and cancers. TIM3 maturation and cell surface expression is facilitated by forming a heterodimeric interaction with CD66a. Co-blockade of CD66a and TIM3 enhanced anti-tumor immune responses, and eliminated tumors in mouse colorectal cancer models.
mRNA expression analysis
Fig 1. RT-PCR analysis of TIM3 gene.
The hTIM3, but not mTIM3, mRNA was detected in splenocytes of the homozygous B-hTIM3 mice.
Protein expression analysis
Fig 2. Spleen cells from both wild type (WT) C57BL/6 and the B-hCD27 homozygous mice were analyzed by flow cytometry.
Mouse TIM3+ cells were detected in non-T and non-B cells from the WT mice, while human TIM3+ cells were detected in non-T and non-B cells from the homozygous B-hTIM3 mice.
Fig 3. Splenocytes from both wild type (WT) C57BL/6 and homozygous B-hTIM3 mice were analyzed by flow cytometry.
Mouse TIM3+ T cells were detected in the WT mice, while human TIM3+ T cells were detected in the homozygous B-hTIM3 mice.
TIM-3 Ab efficacy evaluation
Fig 4. Murine colon cancer MC38 cells were subcutaneously implanted into homozygous B-hTIM3 mice. Mice were divided into control and treatment groups (n = 5) when the tumor volume was about 100 mm3. The anti-human TIM3 antibody significantly inhibited tumor growth in homozygous B-hTIM3 mice, suggesting that the B-hTIM3 mouse model is an effective tool for in vivo hTIM3 antibody efficacy studies. (A) Tumor average volume ± SEM, (B) Mice average weight ± SEM.