CD27 gene function
CD27 (CD27 molecule) is a TNF receptor superfamily member. It is expressed on the surface of T and B cells, and is very important for producing and maintaining long-term T cell immunity. CD27 and its ligand CD70 are combined to provide a co-stimulation signal for T and B cell multiplication and for B cells to produce immunoglobulins. Experiments have demonstrated that a CD27 active type antibody can effectively activate the mouse tumor immune response. Treating mice with a CD27 active type antibody can effectively enhance the antitumor immunity effect for lymph cancer and B16 melanoma, providing putative targets for tumor immunological therapy.
mRNA expression verification
Fig 1. Molecular identification of B-hCD27 humanized mice. mCD27mRNA expression was detected in spleen cells of B-hCD27 heterozygous mice.
Protein expression verification
Fig 2. B-hCD27 humanized mice spleen cell activation and flow cytometry.
mCD27+ cells were detected in C57BL/6 mice and B-hCD27 heterozygous mice. hCD27+ cells were detected in B-hCD27 heterozygous mice.
Fig 3. B-hCD27 mice were used for hCD27 antibody efficacy validation. Mouse colon cancer MC38 cells were subcutaneously transplanted into B-hCD27 heterozygous mice, and animals were grouped into control to treatment groups (n=5) when the tumor size is approximately 100 mm3. Anti-hCD27 antibody shows substantially inhibitory effects. The average±SEM of tumor sizes are shown in the figure. B-hCD27 mice are effective tools for hCD27 antibody efficacy validation.