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B-hTNFRSF18(GITR) mice

2017-08-04 13:36:28.0

Strain description

Strain NameC57BL/6-Tnfrsf18tm1(hTNFRSF18)/BcgenCommon NameB-hTNFRSF18(GITR) mice
BackgroundC57BL/6Catalog numberB-CM-025
Catalog number (Males)BCM025MCatalog number (Females)BCM025F
Related GenesTnfrsf18 (tumor necrosis factor receptor superfamily, member 18)


Gene Description

TNFRSF18 (TNF Receptor Superfamily Member 18) is also known as Glucocorticoid-induced TNFR-related protein (GITR), which is expressed on many immune cells including T cells. As a co-stimulatory signal of T cells, TNFRSF18 is up-regulated upon the activation of T cells, and in turn promotes T cell proliferation. CD25+/CD4+ regulatory T cell is known to mediate immune tolerance, and GITR agonist antibodies can reverse this immune tolerance, and show anti-tumor effect in multiple tumor models.


Targeting strategy

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mRNA expression analysis

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Fig 1. RT-PCR analysis of the GITR gene. 

The mGITR mRNA was detected in both wild type C57BL/6 and heterozygous B-hGITR mice, while the hGITR mRNA was detected only in the heterozygous B-hGITR mice.


Protein expression analysis

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Fig 2. Splenocytes from both wild type (WT) C57BL/6 and heterozygous B-hGITR mice were analyzed by flow cytometry. 

Mouse GITR+ T cells were detected in both WT C57BL/6 and heterozygous B-hGITR mice, while human GITR+ T cells were only detected in the heterozygous B-hGITR mice.



GITR Abs efficacy evaluation

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Fig 3. Murine colon cancer MC38 cells were subcutaneously implanted into heterozygous B-hGITR mice. Mice were grouped when the tumor size was approximately 150±50mm3 (n=6). Three human GITR antibodies differentlially inhibited tumor growth, confirming that the B-hGITR mouse model is a powerful tool for in vivo GITR antibody pharmacological efficacy studies. (A) Tumor average volume ± SEM, (B) Mice average weight ± SEM.

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