|Strain Name||C57BL/6-Tnfrsf18tm1(hTNFRSF18)/Bcgen||Common Name||B-hTNFRSF18(GITR) mice|
|Catalog number (Males)||BCM025M||Catalog number (Females)||BCM025F|
|Related Genes||Tnfrsf18 (tumor necrosis factor receptor superfamily, member 18)|
TNFRSF18 (TNF Receptor Superfamily Member 18) is also known as Glucocorticoid-induced TNFR-related protein (GITR), which is expressed on many immune cells including T cells. As a co-stimulatory signal of T cells, TNFRSF18 is up-regulated upon the activation of T cells, and in turn promotes T cell proliferation. CD25+/CD4+ regulatory T cell is known to mediate immune tolerance, and GITR agonist antibodies can reverse this immune tolerance, and show anti-tumor effect in multiple tumor models.
mRNA expression analysis
Fig 1. RT-PCR analysis of the GITR gene.
The mGITR mRNA was detected in both wild type C57BL/6 and heterozygous B-hGITR mice, while the hGITR mRNA was detected only in the heterozygous B-hGITR mice.
Protein expression analysis
Fig 2. Splenocytes from both wild type (WT) C57BL/6 and heterozygous B-hGITR mice were analyzed by flow cytometry.
Mouse GITR+ T cells were detected in both WT C57BL/6 and heterozygous B-hGITR mice, while human GITR+ T cells were only detected in the heterozygous B-hGITR mice.
GITR Abs efficacy evaluation
Fig 3. Murine colon cancer MC38 cells were subcutaneously implanted into heterozygous B-hGITR mice. Mice were grouped when the tumor size was approximately 150±50mm3 (n=6). Three human GITR antibodies differentlially inhibited tumor growth, confirming that the B-hGITR mouse model is a powerful tool for in vivo GITR antibody pharmacological efficacy studies. (A) Tumor average volume ± SEM, (B) Mice average weight ± SEM.