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B-hCD47 (B/c) mice

2017-08-04 13:37:46.0

Gene Description:

CD47 is an integrin associated protein (IAP) and is an immunoglobulin superfamily member. CD47 is widely expressed on cell surface and interacts with the signal regulatory protein α (SIRPα), thrombospondin-1 (TSP1) and integrins to mediate a series of responses including  apoptosis, proliferation and immunity. CD47 is an important “self” mark on the cell surface and inhibits macrophagocytosis by interaction with SIRPα on macrophage surface. Animal studies have shown that a CD47 antibody is an effective treatment for multiple types of tumors. CD47 is another target for tumor immunity following PD-1/PD-L1.


Mouse background:

BALB/c: inbred strain used for oncology, immunology, neurobiology and inflammation studies, easily produces plasma cell tumors after injection of mineral oil. A variety of mouse tumor cell lines were derived from the BALB/c background, and this strain is good for tumor formation.


Targeting strategy:

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mRNA expression analysis:

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Fig 1. RT-PCR analysis of CD47 gene. The mCD47 mRNA was detected in both wild type BALB/c and heterozygous B-hCD47 (B/c) mice, while the hCD47 mRNA was only detected in the B-hCD47 (B/c) heterozygous mice.


Protein expression analysis:

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Fig 2. Splenocytes from both wild type (WT) BALB/c and heterozygous B-hCD47 (B/c) mice were analyzed by flow cytometry. Mouse CD47+ T cells were detected in both WT BALB/c and heterozygous B-hCD47 (B/c) mice, while human CD47+ T cells were only detected in the heterozygous B-hCD47 (B/c) mice.


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