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B-hPD-1/hTIGIT mice

2017-08-04 13:55:03.0

Strain Description

Strain NameC57BL/6-Pdcd1tm1(hPDCD1)Tigittm1(hTIGIT)/BcgenCommon NameB-hPD-1/hTIGIT mice
BackgroundC57BL/6Catalog numberB-DM-008
Catalog number (Males)BDM008MCatalog number (Females)BDM008F
Related GenesPD-1(Programmed death-1) ;

TIGIT(T-cell immunoreceptor with Ig and ITIM domains)


Targeting Strategy


B-hTIGIT

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B-hPD-1

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mRNA Expression Analysis


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Fig 1. RT-PCR analysis of the B-hPD-1/hTIGIT humanized mice.

The splenocytes of the B-hPD-1/hTIGIT homozygous mice showed expression of human PD-1 and TIGIT mRNA but not mouse PD-1 and TIGIT mRNA.


Protein expression analysis

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Fig 2. Splenocytes from both wild type (WT) C57BL/6 and homozygous B-hPD-1/hTIGIT mice were analyzed by flow cytometry. 

Mouse TIGIT+ T cells were detected in the WT C57BL/6 mice, while human TIGIT+ T cells were detected in the homozygous B-hPD-1/hTIGIT mice.








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Fig 3. Splenocytes from both wild type (WT) C57BL/6 and homozygous B-hPD-1/hTIGIT mice were analyzed by flow cytometry. 

Mouse PD-1+ T cells were detected in the WT C57BL/6 mice, while human PD-1+ T cells were detected in the homozygous B-hPD-1/hTIGIT mice.


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