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B-hPD-1/hTIM3 mice

2017-08-04 13:58:05.0

Strain Description

Strain NameC57BL/6-Pdcd1tm1(hPDCD1)Havcr2tm1(hHAVCR2)/BcgenCommon NameB-hPD-1/hTIM3 mice
BackgroundC57BL/6Catalog numberB-DM-009
Catalog number (Males) BDM009MCatalog number (Females) BDM009F
Related GenesPD-1(Programmed death-1) ;

Havcr2 (hepatitis A virus cellular receptor 2)


Targeting Strategy


B-hTIM3

TIM3打靶策略.png

B-hPD-1

PD-1打靶策略.png

mRNA Expression Analysis


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Fig 1. RT-PCR analysis of the B-hPD-1/hTIM3 humanized mice.

The splenocytes of the B-hPD-1/hTIM3 homozygous mice showed expression of human PD-1 and TIM3 mRNA but not mouse PD-1 and TIM3 mRNA.


Protein expression analysis


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Fig 2. Splenocytes from both wild type (WT) C57BL/6 and homozygous B-hPD-1/hTIM3 mice were analyzed by flow cytometry. 

Mouse TIM3+ T cells were detected in the WT C57BL/6 mice, while human TIM3+ T cells were detected in the homozygous B-hPD-1/hTIM3 mice.







蛋白分析2.png






Fig 3. Splenocytes from both wild type (WT) C57BL/6 and homozygous B-hPD-1/hTIM3 mice were analyzed by flow cytometry. 

Mouse PD-1+ T cells were detected in the WT C57BL/6 mice, while human PD-1+ T cells were detected in the homozygous B-hPD-1/hTIM3 mice.


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