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B-hPD-1/hBTLA mice

2017-08-04 14:00:41.0

Strain Description

Strain NameC57BL/6-Pdcd1tm1(hPDCD1)Btlatm1(hBTLA)/BcgenCommon NameB-hPD-1/hBTLA mice
BackgroundC57BL/6Catalog numberB-DM-002
Catalog number (Males)BDM002MCatalog number (Females)BDM002F
Related GenesPD-1(Programmed death-1) ;

BTLA(B and T lymphocyte associated)


Targeting Strategy


B-hBTLA

BTLA打靶策略.png



B-hPD-1

PD-1 打靶策略.png


mRNA Expression Analysis


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Fig 1. RT-PCR analysis of the B-hPD-1/hBTLA humanized mice.

The splenocytes of the B-hPD-1/hBTLA homozygous mice showed expression of human PD-1 and BTLA mRNA but not mouse PD-1 and BTLA mRNA.


Protein Expression Analysis

蛋白分析1.png







Fig 2. Splenocytes from both wild type (WT) C57BL/6 and homozygous B-hPD-1/hBTLA mice were analyzed by flow cytometry. 

Mouse BTLA+ B cells were detected in the WT C57BL/6 mice, while human BTLA+ B cells were detected in the homozygous B-hPD-1/hBTLA mice.









蛋白分析2.png






Fig 3. Splenocytes from both wild type (WT) C57BL/6 and homozygous B-hPD-1/hBTLA mice were analyzed by flow cytometry. 

Mouse PD-1+ T cells were detected in the WT C57BL/6 mice, while human PD-1+ T cells were detected in the homozygous B-hPD-1/hBTLA mice.


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