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B-hPD-1/h4-1BB mice

2017-08-04 14:01:25.0

Strain Description


Strain NameC57BL/6-Pdcd1tm1(hPDCD1)Cd137tm1(hCD137)/BcgenCommon NameB-hPD-1/h4-1BB mice
BackgroundC57BL/6Catalog numberB-DM-001
Catalog number (Males)BDM001MCatalog number (Females)BDM001F
Related Genes

PD-1(Programmed death-1) ;

TNFRSF9(TNF receptor superfamily member 9, also known as 4-1BB


Targeting Strategy


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mRNA expression analysis


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Fig 1. RT-PCR analysis of the B-hPD-1/h4-1BB humanized mice.

The splenocytes of the B-hPD-1/h4-1BB homozygous mice showed expression of human PD-1 and 4-1BB mRNA but not mouse PD-1 and 4-1BB mRNA.


Protein expression analysis 

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Fig 2.Splenocytes from both wild type (WT) C57BL/6 and homozygous B-hPD-1/h4-1BB mice were analyzed by flow cytometry.

Mouse 4-1BB+ T cells were detected in the WT C57BL/6 mice, while human 4-1BB+ T cells were detected in the homozygous B-hPD-1/h4-1BB mice.









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Fig 3.Splenocytes from both wild type (WT) C57BL/6 and homozygous B-hPD-1/h4-1BB mice were analyzed by flow cytometry.

Mouse PD-1+ T cells were detected in the WT C57BL/6 mice, while human PD-1+ T cells were detected in the homozygous B-hPD-1/h4-1BB mice.


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