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B-hPD-1/hCD27mice

2017-08-04 14:12:43.0

Strain Description


Strain Name C57BL/6-Pdcd1tm1(hPDCD1)Cd27tm1(hCD27)/Bcgen  Common NameB-hPD-1/hCD27 mice
Background  C57BL/6Catalog number B-DM-003
Catalog number (Males) BDM003M Catalog number (Females) BDM003F

Related Genes


PD-1(Programmed death-1) ;

CD27(CD27 molecule)


Targeting Strategy


B-hCD27

CD27打靶策略.png


B-hPD-1

PD-1打靶策略.png


mRNA Expression Analysis


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Fig 1. RT-PCR analysis of the B-hPD-1/hCD27 humanized mice.

The splenocytes of the B-hPD-1/hCD27 homozygous mice showed expression of PD-1 and CD27 mRNA but not mouse PD-1 and CD27 mRNA.


Protein expression analysis

蛋白分析1.png





Fig 2. Splenocytes from both wild type (WT) C57BL/6 and homozygous B-hPD-1/hCD27 mice were analyzed by flow cytometry. 

Mouse CD27+ T cells were detected in both WT  C57BL/6 and the homozygous B-hPD-1/hCD27 mice, while human CD27+ T cells were detected in the homozygous B-hPD-1/hCD27 mice. This might result from the crossrecognition of hCD27 by anti-mCD27 antibodies.





蛋白分析2.png






Fig 3. Splenocytes from both wild type (WT) C57BL/6 and homozygous B-hPD-1/hCD27 mice were analyzed by flow cytometry. 

Mouse PD-1+ T cells were detected in the WT C57BL/6 mice, while human PD-1+ T cells were detected in the homozygous B-hPD-1/hCD27 mice.



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