|Strain Name||C57BL/6-Cd3etm2(hCD3E)/Bcgen||Common Name||B-hCD3e mice(v2)|
|Catalog number (Males)||BCM026M||Catalog number (Females)||BCM026F|
|Related Genes||CD3e (CD3e molecule)|
The protein encoded by CD3e gene is the CD3-epsilon polypeptide, which together with CD3-gamma, -delta and -zeta, and the T-cell receptor alpha/beta and gamma/delta heterodimers, forms the T-cell receptor-CD3 complex. This complex plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways. The genes encoding the epsilon, gamma and delta polypeptides are located in the same cluster on chromosome 11. The epsilon polypeptide plays an essential role in T-cell development.
mRNA expression analysis
Fig 1. RT-PCR analysis of the CD3e gene.
The mCD3e mRNA was detected in both wild t ype C57BL/6 and heterozygous B-hCD3e mice(v2), while the hCD3e mRNA was detected only in the heterozygous B-hCD3e mice(v2).
Protein expression analysis
Fig2. Splenocytes from both wild t ype (WT) C57BL/6 and heterozygous B-hCD3e mice(v2) were analyzed by flow cytometry.
Mouse CD3e+ cells were detected in the WT C57BL/6 mice and heterozygous B-hCD3e mice(v2), while human CD3e+ cells were only detected in the heterozygous B-hCD3e mice(v2).
T cell activation analysis
Fig 3. T cells from both wild t ype (WT) C57BL/6 and heterozygous B-hCD3e (v2) mice were treated with anti-mCD3e antibody (145-2C11) or anti-hCD3e antibody (OKT-3 ) and analyzed by FACS.
T cell activation was detected in both mCD3e-treated WT mice and hCD3e-treated heterozygous B-hCD3e (v2) mice , b ut not in hCD3e-treated WT mice.