中文 | English     

B-hCD3e mice(v2)

2018-02-05 15:23:28.0

Strain description


Strain NameC57BL/6-Cd3etm2(hCD3E)/BcgenCommon NameB-hCD3e mice(v2)
BackgroundC57BL/6Catalog numberB-CM-026
Catalog number (Males)BCM026MCatalog number (Females)BCM026F
Related GenesCD3e (CD3e molecule)


Gene description

The protein encoded by CD3e gene is the CD3-epsilon polypeptide, which together with CD3-gamma, -delta and -zeta, and the T-cell receptor alpha/beta and gamma/delta heterodimers, forms the T-cell receptor-CD3 complex. This complex plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways. The genes encoding the epsilon, gamma and delta polypeptides are located in the same cluster on chromosome 11. The epsilon polypeptide plays an essential role in T-cell development.


Targeting strategy

打靶策略.png

mRNA expression analysis


图1.png


Fig 1. RT-PCR analysis of the CD3e gene.

The mCD3e mRNA was detected in both wild t ype C57BL/6 and heterozygous B-hCD3e mice(v2), while the hCD3e mRNA was detected only in the heterozygous B-hCD3e mice(v2).


Protein expression analysis

图2.png




Fig2. Splenocytes from both wild t ype (WT) C57BL/6 and heterozygous B-hCD3e mice(v2) were analyzed by flow cytometry.

Mouse CD3e+ cells were detected in the WT C57BL/6 mice and heterozygous B-hCD3e mice(v2), while human CD3e+ cells were only detected in the heterozygous B-hCD3e mice(v2).




T cell activation analysis

图3.png

Fig 3. T cells from both wild t ype (WT) C57BL/6 and heterozygous B-hCD3e (v2) mice were treated with anti-mCD3e antibody (145-2C11) or anti-hCD3e antibody (OKT-3 ) and analyzed by FACS. 

T cell activation was detected in both mCD3e-treated WT mice and hCD3e-treated heterozygous B-hCD3e (v2) mice , b ut not in hCD3e-treated WT mice.


Copyright © BIOCYTOGEN LLC. 2017 All Rights Reserved.