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Targeting Vector Construction Services

1) ES Cell based targeting vector: (1-2 months)

Including analysis of gene structure, screening strategy design, targeting vector construction and confirmation (DNA sequencing verification). Southern blot strategy and probe design will also be provided.

2) CRISPR/Cas9 based sgRNA/Cas9 vector: (1-2 months)

Including analysis of gene structure, screening strategy design; construction and confirmation of CRISPR/Cas9 plasmids, sgRNA activity testing (selected the best activity one among 4-6 sgRNAs)

3) CRISPR/Cas9 based donor plasmid (DNA template for cKO/KI): (1-2 months) 

Including analysis of gene structure, screening strategy design; targeting vector construction and confirmation (DNA sequencing verification)

4) Sub-cloning plasmid: (2-3 weeks)


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