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Cell Line Genome Editing

Using the EGE system, EGFP and mCherry genes are respectively knocked into the ACTB and LMNB1 translation initiation sites in the rodent C6 cell lines.

Targeting vector design:

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By using flow cytometry analysis, we have found that the CRISPR/Cas9 mediated gene EGFP-ACTB knockin in efficiency is only 1.91% in U2OS cells while the efficiency is 15.02% by using EGE system, which shows an 8-fold increase. In C6 cell line, the EGFP-LMNB1 knockin efficiency increased from 0.19% to 3.6% by using EGE system, which is about a 19-fold increase.


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