B-CAG-tdTomato cKI mice

C57BL/6-Gt(ROSA)26Sortm1(CAG-tdTomato)Bcgen/Bcgen • 110148

B-CAG-tdTomato cKI mice

Catalog Number: 110148
Strain Name: C57BL/6-Gt(ROSA)26Sortm1(CAG-tdTomato)Bcgen/Bcgen
Strain Background: C57BL/6
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B-CAG-tdTomato cKI mice

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  • Description
  • Targeting strategy
  • Phenotypic analysis

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      Description

      ROSAS26 gene produces a long non-coding RNA (lncRNA) that is under the control of a constitutive promoter. This locus is a widely used site for the integration of transgenes and reporter constructs.

      Gene editing strategy
      A CAG-LSL-tdTomato-WPRE-pA cassette was placed between the exon 1 and exon 2 using C57BL/6 embryonic stem cells. This strain was maintained on a C57BL/6 genetic background.
      Phenotype Analysis

      E

      Experimental design : T cells were isolated from peripheral blood in the single positive mice and the double-positive mice. The proportion of lymphocyte subpopulation were analyzed by flow cytometry.

      Results: tdTomato+ cells were detected in the double-positive mice.

      Experimental design : T cells were isolated from spleen in single positive mice and the double-positive mice. The proportion of lymphocyte subpopulation were analyzed by flow cytometry.

      Results: tdTomato+ cells were detected in the double-positive mice.

      * When publishing results obtained using this animal model, please acknowledge the source as follows: The animal model [B-CAG-tdTomato cKI mice] (Cat# 110148) was purchased from Biocytogen.