Antibody Preparation

Antibody Preparation

Biocytogen carries a variety of professional technical platforms and state-of-art instrumentation. You can expect experienced scientists to perform hybridoma fusion, screening and culturing to ensure high quality at each step of the process. Furthermore, instruments are operated and managed exclusively by well-trained staff to prevent cross-contamination between samples, minimize misoperation occurrence, and ensure normal operation of instruments. For antibody preparation, we provide a detailed product catalog, from which you can browse our data and select desirable antibodies.

Technical Platform

Methods that we are now providing for antibody preparation mainly consist of hybridoma preparation and single cell sequencing. Hybridoma technique is a conventional preparatory approach characterized by immortalized stable clones secreting antigen-specific antibodies, and is thus well received by general antibody R&D staff. However, it has drawbacks i.e. long cycles and genetic heterogeneity. Recently, single-cell sequencing techniques have emerged in the antibody screening field, and it has quickly become one of the most favored methods for modern researchers. As neat as it is revolutionary, single cell sequencing integrates multiple disciplines such as cell biology, molecular biology and bioinformatics.

What makes Biocytogen antibody preparation unique is the RenMab Mouse. Use of an antibody obtained by immunization of RenMab mice allows scientists to skip the humanization process of murine antibodies and obtain fully human antibodies against desired targets in one step, helping you carry out preclinical trials in advance.

 

Hybridoma Preparation

Based on the classical hybridoma technique, this platform optimizes and overcomes technical drawbacks of the conventional technique such as low throughout, long time consumption and complicated operation. Biocytogen has established an in vivo pharmacodynamic screening system for animals, maintaining the advantages of conventional hybridoma technique while overcoming its disadvantages. With our unique screening method, one can obtain high purity hybridoma cells with fewer impurity peaks and good affinity.

Antibody-Discovery-Hybridoma-Preparation

Single Cell Sequencing

Hybridoma cells are fast proliferating and genetically unstable, making antibody yield unpredictable, with many harvested cells unable to secrete antigen-targeting bodies. Biocytogen antibody discovery platform employs advanced single cell sequencing technique to add a unique tag sequence to each cell via a microfluid chip. With a one-time cell bank construction, paired V-region sequences of many cells can be obtained through hybridoma antibody sequencing. Compared with other methods, Biocytogen’s platform has a simple workflow and is able to quickly obtain human or mouse V-region sequence, at the single cell level.

 

RenMab Mouse

Fully Human Antibody Mouse

Hardware Platform

Hybridoma Screening

ClonePix2
ClonePix2
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Automated screening process of ClonePix2 system enables more clones to be screened in a shorter time. Based on expression level of clone-secreted antibody, ClonePix2 sorts clones automatically, thereby enabling users to select high-expression clones. Culturing cells with semisolid medium enables harvest of a single clone of hybridoma or CHO-K1 cells. It is simple and convenient to operate, capable of avoiding shortcomings such as time-consuming limiting dilution and few positive clones, while the selection does not affect cell viability and proliferation capacity.

ClonePix2-Map
ClonePix2-Cells

Figure 2. ClonePix2 locates and selects accurately hybridoma cell clones (pointed at by yellow arrows) with high antibody expression

Intellicyt iQue Plus
Intellicyt-iQue-Plus
Intellicyt iQue Plus
CloneSelect Imager
CloneSelect-Imager
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Antibody Expression and Purification

QuantStudio® 5 real-time fluorescence-based quantitative PCR system
Quant-Studio
AKTA avant 150
AKTA-Avant-150
ambr® 250 modular
Ambr-250-Modular
ISF1-X cell shaker
ISF-1-X-Cell-Shaker
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Biological and Physiochemical Analysis

Biacore 8K & T200
Biacore-8K
Agilent 1260 Infinity liquid chromatograph system
Agilent-1260
WATERS XEVO G2-XS UPLC-QTOF
Waters-Xevo-G2-XS
Maurice CE-SDS+ iCIEF dual function analysis system
Maurice-CE-SDS
SpectraMax Paradigm multimode plate reader
SpectraMax
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Product Catalog

Immune Checkpoint

OX40 CD40 4-1BB GITR
CD28 ICOS CD3e CD27
CTLA4 CD47 PD-L1 PD-1 TIGIT
BTLA SIRPα Tim3 B7-H3 LAG3
VISTA PD-L2

Cytokines

IL-1A IL-1B IL-1RN IL2RA IL-3 IL-3RA
IL4 IL4R IL-5 IL-5RA IL6 IL6R
IL-6RB IL-7 IL-8 IL-9 IL-10 IL-11
IL-12A IL-12B IL-12Rβ1 IL-12Rβ2 IL-13 IL-15
IL17A IL17RA IL-17F IL-17RC IL-18 IL-21
IL-22 IL-23A IL-23R IL-27A IL-27RA IL-31
IL-31RA IL-33 IL-33R IL-36R GM-CSF GM-CSFR
IL1RACP

Others

dogPD1 dogTNFα CD14 CD16A CD19 CD20
CD27L CD38 CD39 CD48 CD70 CD83
CD73 CD112 CD155 CD161 CD205 CD226
CD244 BCMA BDCA-2 BDCA-3 CCR5 CEACAM1
Ckit CLEC9A CSF1R CX3CL1 GCGR FGL1
LAIR1 LTBR MERTK NKG2A NKG2D NKP30
NKP44 NKP46 TSLP VEGFA 41BBL
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