Biocytogen carries a variety of professional technical platforms and state-of-art instrumentation. You can expect experienced scientists to perform hybridoma fusion, screening and culturing to ensure high quality at each step of the process. Furthermore, instruments are operated and managed exclusively by well-trained staff to prevent cross-contamination between samples, minimize misoperation occurrence, and ensure normal operation of instruments. For antibody preparation, we provide a detailed product catalog, from which you can browse our data and select desirable antibodies.
Methods that we are now providing for antibody preparation mainly consist of hybridoma preparation and single cell sequencing. Hybridoma technique is a conventional preparatory approach characterized by immortalized stable clones secreting antigen-specific antibodies, and is thus well received by general antibody R&D staff. However, it has drawbacks i.e. long cycles and genetic heterogeneity. Recently, single-cell sequencing techniques have emerged in the antibody screening field, and it has quickly become one of the most favored methods for modern researchers. As neat as it is revolutionary, single cell sequencing integrates multiple disciplines such as cell biology, molecular biology and bioinformatics.
What makes Biocytogen antibody preparation unique is the RenMab Mouse. Use of an antibody obtained by immunization of RenMab mice allows scientists to skip the humanization process of murine antibodies and obtain fully human antibodies against desired targets in one step, helping you carry out preclinical trials in advance.
Based on the classical hybridoma technique, this platform optimizes and overcomes technical drawbacks of the conventional technique such as low throughout, long time consumption and complicated operation. Biocytogen has established an in vivo pharmacodynamic screening system for animals, maintaining the advantages of conventional hybridoma technique while overcoming its disadvantages. With our unique screening method, one can obtain high purity hybridoma cells with fewer impurity peaks and good affinity.
Single Cell Sequencing
Hybridoma cells are fast proliferating and genetically unstable, making antibody yield unpredictable, with many harvested cells unable to secrete antigen-targeting bodies. Biocytogen antibody discovery platform employs advanced single cell sequencing technique to add a unique tag sequence to each cell via a microfluid chip. With a one-time cell bank construction, paired V-region sequences of many cells can be obtained through hybridoma antibody sequencing. Compared with other methods, Biocytogen’s platform has a simple workflow and is able to quickly obtain human or mouse V-region sequence, at the single cell level.
Fully Human Antibody Mouse
Hybridoma ScreeningClonePix2Heading layer
Automated screening process of ClonePix2 system enables more clones to be screened in a shorter time. Based on expression level of clone-secreted antibody, ClonePix2 sorts clones automatically, thereby enabling users to select high-expression clones. Culturing cells with semisolid medium enables harvest of a single clone of hybridoma or CHO-K1 cells. It is simple and convenient to operate, capable of avoiding shortcomings such as time-consuming limiting dilution and few positive clones, while the selection does not affect cell viability and proliferation capacity.
Figure 2. ClonePix2 locates and selects accurately hybridoma cell clones (pointed at by yellow arrows) with high antibody expressionIntellicyt iQue PlusIntellicyt iQue PlusCloneSelect Imager
Antibody Expression and PurificationQuantStudio® 5 real-time fluorescence-based quantitative PCR systemAKTA avant 150ambr® 250 modularISF1-X cell shaker
Biological and Physiochemical AnalysisBiacore 8K & T200Agilent 1260 Infinity liquid chromatograph systemWATERS XEVO G2-XS UPLC-QTOFMaurice CE-SDS+ iCIEF dual function analysis systemSpectraMax Paradigm multimode plate reader
OX40 CD40 4-1BB GITR CD28 ICOS CD3e CD27 CTLA4 CD47 PD-L1 PD-1 TIGIT BTLA SIRPα Tim3 B7-H3 LAG3 VISTA PD-L2
IL-1A IL-1B IL-1RN IL2RA IL-3 IL-3RA IL4 IL4R IL-5 IL-5RA IL6 IL6R IL-6RB IL-7 IL-8 IL-9 IL-10 IL-11 IL-12A IL-12B IL-12Rβ1 IL-12Rβ2 IL-13 IL-15 IL17A IL17RA IL-17F IL-17RC IL-18 IL-21 IL-22 IL-23A IL-23R IL-27A IL-27RA IL-31 IL-31RA IL-33 IL-33R IL-36R GM-CSF GM-CSFR IL1RACP
dogPD1 dogTNFα CD14 CD16A CD19 CD20 CD27L CD38 CD39 CD48 CD70 CD83 CD73 CD112 CD155 CD161 CD205 CD226 CD244 BCMA BDCA-2 BDCA-3 CCR5 CEACAM1 Ckit CLEC9A CSF1R CX3CL1 GCGR FGL1 LAIR1 LTBR MERTK NKG2A NKG2D NKP30 NKP44 NKP46 TSLP VEGFA 41BBL