GAN model

Dietary components include high fructose and high cholesterol (40 kcal% fat, mainly palm oil, 20 kcal% fructose and 2% cholesterol), and this diet-induced NASH model develops obesity, impaired glucose tolerance and hepatic steatosis.

Dietary components include high fructose and high cholesterol (40 kcal% fat, mainly palm oil, 20 kcal% fructose and 2% cholesterol), and this diet-induced NASH model develops obesity, impaired glucose tolerance and hepatic steatosis.

Gubra-Amylin diet-induced NASH model in 6-week-old C57BL/6 male mice

Gubra-Amylin diet-induced NASH model in 6-week-old C57BL/6 male mice. (A) Body weight change after treatment. (B) Glucose tolerance ability after treatment. (C) The area under the curve of B. (D) Representative pictures of H&E staining after 20 weeks of induction. (E) NAFLD activity score (NAS) was assessed by an external expert pathologist. Dulaglutide treatment improved GAN diet-induced NASH. Data are expressed as mean ± SEM, n=9 mice per group. *p<0.05, **p<0.01,***p<0.001.

Gubra-Amylin diet-induced NASH model in aged mice

Gubra-Amylin diet-induced NASH model in aged mice. (A-B) Increased body weight in Gubra-Amylin diet-induced group. (C) Impaired glucose tolerance ability induced by the Gubra-Amylin diet. (D) The area under the curve of C. (E) Increased plasma insulin content in the Gubra-Amylin diet-induced group. Data is represented as mean ± SEM, n=6-10 mice per group. *P <0.05, **P <0.01, ***P <0.001.

 

Gubra-Amylin diet-induced NASH in aged mice. (A) Representative pictures of H&E staining after 12 weeks of induction. (B) NAFLD activity score (NAS) was assessed by an external expert pathologist. N=6-10 mice per group. Data are expressed as mean ± SEM. **p<0.01.

Gubra-Amylin diet-induced fibrosis in aged mice

Gubra-Amylin diet-induced fibrosis in aged mice. (A) Representative pictures of Sirius red staining after 21 weeks of induction. (B) Fibrosis score of Sirius red staining. Aged mice fed a GAN diet showed significantly increased fibrosis. n=6-10 mice per group. Data are expressed as mean ± SEM. **p<0.01.

Gubra-Amylin diet-induced NASH model in B-ob/ob mice

Gubra-Amylin diet-induced NASH model in B-ob/ob mice. (A) Body weight change under treatment. (B) Glucose tolerance ability after treatment. (C) The area under the curve of B. Data are expressed as mean ± SEM. N=9 mice per group. *p<0.05, **p<0.01,***p<0.001.

Gubra-Amylin diet-induced NASH and fibrosis in B-ob/ob mice

Gubra-Amylin diet-induced NASH and fibrosis in B-ob/ob mice.

(A) Representative pictures of H&E staining after 16 weeks of induction. (B) NAFLD activity score (NAS) was assessed by an external expert pathologist. (C) Representative pictures of Sirius red staining after 16 weeks of induction. (D) Fibrosis score of Sirius red staining. Data are expressed as mean ± SEM. N=9 mice per group.

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A new NASH model in aged mice with rapid progression of steatohepatitis and fibrosis

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