HFMCD induced NASH Model

With a strong focus on immuno-oncology applications, we provide translational in vivo efficacy studies, PK/PD, toxicity studies, and in vitro pharmacology support.

The high-fat methionine-choline-deficient diet (HFMCD) is a classic model for NASH induction, which consists of a 60 kcal% fat, methionine and choline-deficient diet. Both methionine and choline can promote the delivery of fat from the liver through the blood in the form of phospholipids, improve the utilization of fatty acids in the liver, and prevent the abnormal accumulation of liver fat. HFMCD-induced NASH mice display increased liver injury, hepatic steatosis, and fibrosis accompanied by increased NAS scores.

High-Fat Methionine-Choline-Deficient Diet (HFMCD)-Induction of NASH

A model of NASH induced by a high-fat methionine-choline-deficient diet (HFMCD). Wild-type C57BL/6J mice were randomly divided into four groups and given a standard diet (STD) and a high-fat methionine-choline-deficient (HFMCD) diet and fed for 4 weeks and 6 weeks to detect various parameters. (A) HFMCD-induced NASH model protocol diagram. (B) Mouse body weight, (C) liver weight, (D) ratio of liver weight to body weight, (E-F) serum ALT and AST concentrations, (G-H) H&E staining and NAS scores of liver tissue sections, (I-J) Sirius red staining of liver tissue sections and quantification statistics of the degree of liver fibrosis were measured in mice fed a STD or HFMCD. The results showed that compared to the control group, increased liver weight and serum ALT and AST concentrations were observed in mice fed a HFMCD. Additionally, H&E staining of liver tissue sections showed extensive hepatocyte steatosis, ballooning degeneration and intralobular inflammation, while Sirius red staining of liver tissue sections showed significant liver fibrosis. These results suggest that HFMCD induction can successfully establish a NASH mouse model. Data is represented as mean ± SEM, n=5.

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