Conditional Knockout

Conditional Knockout

A conditional Knockout (cKO) is usually generated through the Cre-LoxP/Flp-Frt recombination systems. The candidate fragment that will be knocked out is flanked by LoxP or Frt elements. The floxed (flanked by LoxP or Frt) mice are bred with tissue-specific Cre (or Flp) mice, so that the flanked sequence will be removed from the offsprings’ genome in a tissue-specific pattern. For the conditional knockout generation, the loxP fragments are usually inserted into the introns downstream of the ATG-containing exon, and removal of the flanked exon(s) will result in a frame-shift that disrupts protein expression.

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