During feeding, mice may be contaminated by different microbial pathogens in the environment; some embryonic biological techniques can be used to rederivate such mice into SPF grade or even higher grade mice; at present, Biocytogen rederivates foreign non-SPF grade mice by IVF.
Specifications for IVF Mouse Rederivation Services
Acceptance Criteria for Parental Mice
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- Parental mice to be provided by the client: 3–5 fertile male mice (10–20 weeks of age).
- Documents to be filled by the client: 1. Genotype identification protocol for the mice to be rederivated (Biocytogen BD will send the protocol to the client). 2. Based on the microbial testing report for animals in the last 3 months, animals other than those tested positive for 7 assays can be accepted for rederivation (see Table 1).
Table 1. Biocytogen tests of rederivated parental mice and acceptance criteria for parental mice
| Category | No. | Test Item | Acceptance Criteria for Parental Mice | Remarks |
| Mouse bacteriology | 1 | Salmonella spp. | All 8 tests are required according to relevant national standard | |
| 2 | Corynebacterium kutscheri | Do not accept if it is tested positive | ||
| 3 | Mycoplasma pneumoniae | |||
| 4 | Pasteurella pneumotropica | |||
| 5 | Klebsiella pneumoniae | |||
| 6 | Staphylococcus aureus | |||
| 7 | Pseudomonas aeruginosa | |||
| 8 | Clostridium piliforme | |||
| Mouse serology | 1 | Sendai Virus | Do not accept if it is tested positive | All 6 tests are required according to relevant national standard |
| 2 | Pneumonia Virus of Mice | Do not accept if it is tested positive | ||
| 3 | Mouse Hepatitis Virus | Do not accept if it is tested positive | ||
| 4 | Minute Virus of Mice | Do not accept if it is tested positive | ||
| 5 | Reovirus 3 | Do not accept if it is tested positive | ||
| 6 | Ectromelia Virus | Do not accept if it is tested positive | ||
| Mouse parasitology | 1 | In vitro parasites (arthropods) | All 5 tests are required according to relevant national standard | |
| 2 | Toxoplasma Gondii | |||
| 3 | Enteric Helminths | |||
| 4 | Flagellates | |||
| 5 | Ciliates |
Specification for the Delivery of Rederivated Mice
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- Quantity of delivered mice: Not fewer than 2 pairs of positive mice depending on the customer’s target.
- Genotype of delivered mice: Consistent with the genotype required by the client.
- Microbial level of delivered mice: Tested negative for all 45 tests (see Table 2).
- Accompanying documents submitted at the time of mouse delivery: 1. Mouse genotype identification report. 2. Microbial testing report for mice (An analysis report for corresponding tests will be provided upon request of the client, and testing cost will be charged separately depending on test specifics).
Table 2. Pathogen Exclusion List in Biocytogen Animal Facility
| No. | Test Item |
| 1 | Salmonella spp. |
| 2 | Corynebacterium kutscheri |
| 3 | Pasteurella pneumotropica |
| 4 | Klebsiella pneumoniae |
| 5 | Staphylococcus aureus |
| 6 | Pseudomonas aeruginosa |
| 7 | Ectoparasites |
| 8 | Ectromelia Virus |
| 9 | Mouse Hepatitis Virus (MHV) |
| 10 | Sendai Virus |
| 11 | Pneumonia Virus of Mice (PVM) |
| 12 | Reovirus 3 (Reo 3) |
| 13 | Minute Virus of Mice |
| 14 | Clostridium piliforme |
| 15 | Lymphocytic Choriomeningitis Virus |
| 16 | Hantaan Virus |
| 17 | Mouse Encephalomyelitis Virus(GDVII)/Rat Theilovirus (RTV) |
| 18 | Mouse Adenovirus I and II (FL and K88) |
| 19 | Polyoma Virus |
| 20 | Streptococcus pneumoniae |
| 21 | Encephalitozoon cuniculi |
| 22 | Beta hemolytic Streptococcus (non-Group D) |
| 23 | Mycoplasma spp |
| 24 | Mycoplasma Pulmonis |
| 25 | Enteric Helminths |
| 26 | Endoparasites |
| 27 | Ciliates |
| 28 | Toxoplasma |
| 29 | Bordetella bronchiseptica |
| 30 | Pasteurella multocida |
| 31 | Klebsiella oxytoca |
| 32 | Citrobacter rodentium |
| 33 | Proteus spp. |
| 34 | Mouse Parvovirus (MPV) |
| 35 | Murine Norovirus (MNV) |
| 36 | Mouse Rotavirus (EDIM) |
| 37 | K Virus |
| 38 | Mouse Cytomegalovirus (MCMV) |
| 39 | Cilia Associated Respiratory Bacillus |
| 40 | Thymic Virus |
| 41 | Lactate Dehydrogenase Elevating Virus |
| 42 | Helicobacter spp. |
| 43 | Streptococcus B-hemolytic (Groups B) |
| 44 | Streptococcus B-hemolytic (Groups G) |
| 45 | NS-1 |
