AACR 2022: Evaluating In Vivo Efficacy of Bi-Functional Fusion Protein Anti-PD-1-IL21 in Humanized B-hIL21R Mice and B-hPD-1/hIL21R Mice
- mRNA expression analysis:
Murine Il21r mRNA was detected in splenocytes isolated from wild-type mice, while human IL21R mRNA was detected only in humanized B-hIL21R mice.
- Protein expression analysis:
Murine IL21R protein was detected in wild-type mice, while human IL21R protein was exclusively detected in humanized B-hIL21R mice.
- Leukocyte cell subpopulation analysis:
IL21R humanization does not change the overall development, differentiation or distribution of immune cell types in the spleen, lymph node or blood.
- Functional analysis:
STAT3 phosphorylation was successfully induced with mouse IL21 or human IL21 in humanized B-hIL21R mice.
- Antibody efficacy evaluation:
Human IL21-Fc was effective in controlling tumor growth in B-hIL21R mice, demonstrating that the B-hIL21R mice provide a powerful preclinical model for in vivo evaluation of IL21 cytokine or fusion protein drugs.
Related mouse models: humanized B-hIL21R mice