AACR 2023: Generation of Humanized TREM2 Mice for Preclinical Evaluation of Therapeutics Targeting Tumor-Associated Macrophages

AACR 2023: Generation of Humanized TREM2 Mice for Preclinical Evaluation of Therapeutics Targeting Tumor-Associated Macrophages

Used in the poster: B-hTREM2 mice

Conclusions

  • Murine Trem2 mRNA was detected in wild-type mice, while human TREM2 mRNA was detected in homozygous B-hTREM2 mice by RT-PCR.
  • TREM2 protein was detected in microglia and tumor-derived macrophages isolated from wild-type and homozygous B-hTREM2 mice by flow cytometry.
  • Human TREM2 protein was detected in B-hTREM2 mice by western blot using an anti-human TREM2 antibody.
  • Anti-human TREM2 antibody (analog) was effective in controlling MC38 and EMT-6 tumor growth in B-hTREM2 mice.

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