mRNA expression analysis:

Mouse Ox40l mRNA was detected in dendritic cells isolated from wild-type mice, while human OX40L mRNA was detected in homozygous B-hOX40/hOX40L mice by RT-PCR. Protein expression analysis:

Human OX40 and OX40L protein expression was detected in B-hOX40/hOX40L mice by flow cytometry. Immune cell analysis:

OX40 and OX40L humanization does not change the overall development, differentiation or distribution of spleen, lymph node, and blood immune cells in B-hOX40/hOX40L mice.