Psoriasis Model

Psoriasis Model

Introduction

Psoriasis is a type of skin disease characterized by hyperproliferation of the epidermal keratinocytes. It is thought to be mediated by cells and molecules of both the innate and adaptive immune systems, as a result of a combination of genetic, epigenetic, and environmental influences. The most commonly used animal model is the imiquimod (IMQ)-induced skin lesion and skin inflammation, which phenotypically resembles human psoriasis.

The degree of imiquimod-induced skin injury in this murine model, with symptoms of skin rashes and peeling in mice, can be monitored through a standard clinical score system. The cutaneous epidermis is thickened. Parakeratosis and inflammatory infiltration of leukocytes predominantly into dermis can be seen histopathologically. The IL-23/IL-17 axis is believed to be particularly important in psoriasis pathogenesis due to its pro-inflammatory effects. IL-17 cytokine level is elevated in the imiquimod-induced psoriasis model.

Biocytogen provides imiquimod-induced psoriasis model for your preclinical study of psoriasis drug discovery. In particular, we generated B-hIL17A mice where the human IL-17A gene is knocked-in and replaces the mouse IL-17A gene, thus providing a genetically humanized mouse psoriasis model for convenient testing of therapeutics targeting the human IL-17A.

Generation of B-hIL17A Mice and its phenotypic analysis

Generation of Imiquimod-Induced Psoriasis Model and Its Phenotypic Analysis

IMQ-induced psoriasis model

IMQ-Induced-Model

IMQ cream was applied topically on left ear and back, QD x 15.

Ear thickness and clinical score in psoriasis model in C57BL/6 mice

Ear-Thickness

Mice (n=5) were scored daily for up to 8 days for clinical signs of skin inflammation following treatment with either control vaseline or IMQ cream (A-D). Results showed that IMQ significantly increased clinical signs of skin inflammation. Ear thickness was also increased by IMQ treatment (E).

IMQ-Increased-Thickness

IMQ increased thickness of sections of ear and back skin of mice treated with IMQ cream (H&E). B. IMQ increased skin thickness measurement. Five random points were selected and measured. The results showed that IMQ significantly increased epidermal thickness in ear and back skin. Scale bar: 100 um.

Ear thickness and clinical score in psoriasis model in B-hIL17A mice

Ear-Thickness-and-Clinical-Score

Mice (n=5) were scored daily for up to 8 days for clinical signs of skin inflammation following treatment with either control vaseline or IMQ cream (A-D). Results showed that IMQ significantly increased clinical signs of skin inflammation. Ear thickness was also increased by IMQ treatment (E).

IMQ-Cell

IMQ increased thickness of sections of ear and back skin of mice treated with IMQ cream (H&E). B. IMQ increased skin thickness measurement. Five random points were selected and measured. The results showed that IMQ significantly increased epidermal thickness in ear and back skin. Scale bar: 100 um.

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