Inflammation and Autoimmunity

IBD Mouse Model

Biocytogen has developed DSS mouse model, TNBS mouse model, and T cell transfer IBD mouse models, along with various IBD-related target humanized mice (e.g.B-hTL1A mice), for preclinical drug evaluation, complete with full datasets.

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    IBD Mouse Model

    Inflammatory bowel disease (IBD), including ulcerative colitis (UC) and Crohn's disease (CD), is a chronic inflammatory disorder affecting the gastrointestinal tract. Clinical symptoms often include recurrent diarrhea, abdominal pain, intestinal bleeding /hematochezia, fever, and weight loss. Although the exact pathogenesis remains unclear, factors such as genetics, immune dysregulation, environmental triggers, and gut microbiota are all associated with disease development.

    To study IBD mechanisms and evaluate treatment efficacy, researchers commonly use preclinical IBD mouse models. One of the most widely used models is chemically induced using dextran sulfate sodium (DSS)—the DSS colitis model. When DSS is administered through drinking water, it damages the intestinal epithelial barrier, activates non-specific immune cells to release cytokines, and disrupts mucosal integrity. These changes result in clinical and pathological features in mice—such as weight loss, loose stools, hematochezia, and granulocyte infiltration—that closely resemble human ulcerative colitis.

    Biocytogen has developed a stable and reproducible DSS-induced IBD mouse model using C57BL/6 mice, providing a reliable tool for preclinical IBD research and pharmacodynamic evaluation of anti-inflammatory therapies. Biocytogen's humanized mouse models (e.g. B-hTL1A mice) for inflammatory bowel disease (IBD) stably express fully human TL1A protein, providing a precise preclinical testing platform for drug development that is relevant to human IBD.

    DSS-induced IBD Mouse Model
    Establishment of IBD Mouse Models
    Mice strains
    B-hCXCR2
    B-hTL1A
    B-hTL1A/IL23A/IL12B
    Readout
    Included tests Clinical scores Body weight
    DAI score
    Colon Colon length
    Colon weight
    Histopathology H&E
    Optional tests Tissue homogenate Cytokines test
    Tissue histopathology IHC
    Efficacy Validation on IBD Mouse Models in C57BL/6 for CsA (cyclosporin A)

    Efficacy validation on IBD mouse models for CsA (cyclosporin A). C57BL/6 mice were provided drinking water containing DSS for 7 consecutive days, and body weight changes were recorded throughout and scored clinically (A-D) at study endpoint, the colon weight and colon length were recorded. Two way-ANOVA (A-D) or one way ANOVA (E-F) followed by multiple comparison. Values are expressed as mean ± SEM. ****p<0.0001, ***p<0.001 , **p<0.01 , *p<0.05.

    Histologic assessment of the colon of IBD mouse model

    Histologic assessment of DSS-induced colitis in IBD mouse models. Photomicrographs of histopathological score and H&E staining cross-sections (10x and 40x magnification). Values are expressed as mean ± SEM. ****p<0.0001, ***p<0.001 , **p<0.01 , *p<0.05

    Anti-IL12/23p40 (mouse) alleviated DSS-induced acute colitis

    Effects of anti-IL12/23p40 on DSS-induced acute colitis.
    C57BL/6 mice received 3% DSS in drinking water for 5 days, CsA was injected to mice every day from day0 to day7, anti-IL12/23P40 was administered to mice on day2, day 6 and day 8 (A). Body weight and excreta were recorded every day(B, C). At the end, colon was collected for H&E staining, colon length (D) and weight (E) were recorded. CsA and anti-IL12/23p40 alleviated 3% DSS induced ulcerative colitis in this experiment. Two way-ANOVA followed by multiple comparison. Values are expressed as mean ± SEM. ****p<0.0001, ***p<0.001 , **p<0.01 , *p<0.05.

    Anti-CXCR2 alleviated DSS-induced acute colitis on CXCR2 humanized mice (B-hCXCR2 mice)

    Efficacy validation on IBD(B-hCXCR2 mice) mouse models
    C57BL/6 mice and B-hCXCR2 mice were provided drinking water containing DSS for 7 consecutive days, after which normal drinking water resumed. Body weight changes and clinical scores ( Weight loss score, stool hardness score, blood in stool score as well as total DAI score) were recorded throughout. Two way-ANOVA followed by multiple comparison, all group compared with G3. Values are expressed as mean ± SEM. ****p<0.0001, ***p<0.001 , **p<0.01 , *p<0.05.

    Anti-CXCR alleviated DSS-induced acute colitis on CXCR2 humanized mice (B-hCXCR2 mice)

    Gross sampling and colon evaluation and histologic assessment of the colon of IBD mouse model. C57BL/6 mice and B-hCXCR2 mice were provided drinking water containing DSS for 7 consecutive days, after which normal drinking water resumed. At study endpoint, colon length (A) and colon weight (B) were measured. Colon tissue were used for H&E staining (C). One-way ANOVA followed by multiple comparison, all group compared with G3. Values are expressed as mean ± SEM.

    DSS-induced acute colitis on TL1A/IL23A/IL12B humanized mice (B-hTL1A/IL23A/IL12B mice)

    C57BL/6 mice and B-hTL1A/IL23A/IL12B mice were provided drinking water containing different concentration of DSS for 7 consecutive days. Body weight changes and clinical scores (Weight loss score, stool hardness score, blood in stool score as well as total DAI score) were recorded throughout. Two way-ANOVA followed by multiple comparison, all group compared with G1. Values are expressed as mean ± SEM. ****p<0.0001, ***p<0.001 , **p<0.01 , *p<0.05.

    DSS-induced acute colitis on TL1A/IL23A/IL12B humanized mice (B-hTL1A/IL23A/IL12B mice)

    C57BL/6 mice and TL1A/IL23A/IL12B humanized mice (B-hTL1A/IL23A/IL12B mice) were provided drinking water containing different concentration of DSS for 7 consecutive days. At study endpoint, colon length and colon weight were measured. And colon tissue were used for H&E staining. One way ANOVA followed by multiple comparison compared to G1. Values are expressed as mean ± SEM. ****p<0.0001, ***p<0.001 , **p<0.01 , *p<0.05.