B-CAG-hHER2 MC38 Cell Line

Basic Information

Strain Name
MC38 Cd274 tm2(CAG-CD274)Bcgen
Stock Number
310701
Common Name
B-CAG-hHER2 MC38 Cell Line
Disease
Colorectal cancer
Species
Mus musculus, mouse (C57BL/6J)
Growth factors
Adherent
Culture medium
DMEM+10%FBS
Frozen
90% Complete medium+10% DMSO

Targeting Strategy

B-CAG-hHER2-MC38-Cell-Line-targeting-strategy

Details

Phenotyping Information

Phenotypic analysis of B-CAG-hHER2 MC38 Cell Line

B-CAG-hHER2-MC38-Cell-Line-details-Phenotypic-analysis

B-CAG-hHER2 MC38 Cell Line were analyzed by flow cytometry. The hHER2, but not mHER2, protein was detected in B-CAG-hHER2 MC38 Cell Line.

Tumor Growth Curve

B-CAG-hHER2-MC38-Cell-Line-details-tumor-growth-curve

C57BL/6 mice were subcutaneously injected with tumor cells in the right front flank for tumor development. Tumor size was measured two times weekly in two dimensions using a caliper, and the volume was expressed in mm3 using the formula: V=0.5a X b 2 where a and b were the long and short diameters of the tumor, respectively.

 

Tumor cells analysis

Gating strategy

B-CAG-hHER2-MC38-Cell-Line-details-tumor-cells-analysis-gating-strategy

Tumor cells analysis

B-CAG-hHER2-MC38-Cell-Line-details-tumor-cells-analysis-tumor-cells-analysis

B-CAG-hHER2 MC38 and MC38 tumors were analyzed by flow cytometry. Results shows that compared with MC38, the expression of hHER2 on the surface of B-CAG-hHER2 MC38 tumor cells was significantly increased.

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