Phenotypic analysis of B-CAG-hPD-L2 MC38 Cell Line
B-CAG-hPD-L2 MC38 Cell Line were analyzed by flow cytometry. The hPD-L2, but not mPD-L2, protein was detected in B-CAG-hPD-L2 MC38 Cell Line.
Tumor Growth Curve
C57BL/6 mice were subcutaneously injected with tumor cells (5E5) in the right front flank for tumor development. Tumor size was measured two times weekly in two dimensions using a caliper, and the volume was expressed in mm3 using the formula: V=0.5a X b2, where a and b were the long and short diameters of the tumor, respectively.
Tumor Cells Analysis
B-CAG-hPD-L2 MC38 and MC38 tumors were analyzed by flow cytometry. Results shows that compared with MC38, the expression of hPD-L2 on the surface of B-CAG-hHER2 MC38 tumor cells was significantly increased.