B-Drd2-Cre Rats

Basic Information

Strain Name
SD-Drd2 tm1(Cre) /Bcgen
Stock Number
B-CR-003
Common Name
B-Drd2-Cre rats
Source/Investigator
Bcgen (Beijing Biocytogen Co., Ltd)
Related Genes
Drd2 (dopamine receptor D2); D2R; Drd-2
Species
Sprague Dawley
Appearance
White
Genotypes
Drd2 Cre (wt/mut)
Development
A P2A-NLS-Cre sequence cassette was placed between the coding sequence of exon 3 and 3’UTR of the Drd2 gene using CRISPR/Cas9 technology. This strain was maintained on a Sprague Dawley genetic background.

Description

The Drd2 gene encodes dopamine receptor D2. In this strain, Cre recombinase expression is under the control of Drd2 promoter. When crossed with a strain containing a loxP site-flanked sequence of interest, Cre-mediated recombination results in deletion of the flanked sequence in Drd2 expressing cells.

Targeting Strategy

Details

Genotyping Information

Primer Sequence (5’-3’) Tm
(℃)
Product
size (bp)
Drd2 Cre primer 1 ACCTAGTCCAGTCTTTCCTTCGCCT 62 Mut: 407
Drd2 Cre primer 2 CGATCCCTGAACATGTCCATCAG 58
Drd2 Cre primer 1 ACCTAGTCCAGTCTTTCCTTCGCCT 62 Mut: 456
WT: 1551
Drd2 Cre primer 3 AGATACCAGTCTCCCTGGCCCTAC 62

Polymerase: Kod-plus

 

References

1.Bunzow, J. R., Van Tol, H. H., Grandy, D. K., Albert, P., Salon, J., Christie, M., Machida, C.
A., Neve, K. A., Civelli, O. Cloning and expression of a rat D2 dopamine receptor
cDNA. Nature 336: 783-787, 1988. [PubMed: 2974511]
2. Basu, S., Nagy, J. A., Pal, S., Vasile, E., Eckelhoefer, I. A., Bliss, V. S., Manseau, E. J.,
Dasgupta, P. S., Dvorak, H. F., Mukhopadhyay, D. The neurotransmitter dopamine inhibits angiogenesis induced by vascular permeability factor/vascular endothelial growth factor. Nature Med. 7: 569-574, 2001. [PubMed: 11329058]
3.Duan, J., Wainwright, M. S., Comeron, J. M., Saitou, N., Sanders, A. R., Gelernter, J.,
Gejman, P. V. Synonymous mutations in the human dopamine receptor D2 (DRD2) affect
mRNA stability and synthesis of the receptor. Hum. Molec. Genet. 12: 205-216, 2003. [PubMed: 12554675]
4. Balk, J.-H., Picetti, R., Salardi, A., Thirlet, G., Dierich, A., Depaulls, A., Le Meur, M.,
Borrelli, E. Parkinsonian-like locomotor impairment in mice lacking dopamine D2
receptors. Nature 377: 424-428, 1995. [PubMed: 7566118, related citations]

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