B-hPD-L1 (CD274) Mice

Basic Information

Strain Name
C57BL/6-Cd274tm1(CD274)Bcgen/Bcgen
Stock Number
110015
Common Name
B-hPD-L1 (CD274) Mice
Source/Investigator
Bcgen (Beijing Biocytogen Co., Ltd)
Related Genes
Cd274 (CD274 antigen)
Species
C57BL/6
Appearance
Black
Genotypes
Homozygous

Description

PD-L1 (Programmed cell death ligand-1), also known as B7-H1 and CD274, is mainly expressed in antigen-presenting cells (APCs) and activated T cells and is one of the two ligands of PD-1. The interaction between PD1 and PD-L1 plays an important role in the negative regulation of the immune response. PD-L1 is highly expressed in a variety of solid tumors. PD-1 and PD-L1 interactions can reduce T cell activation and promote tumor immune escape. The PD-1/PD-L1 signaling pathway can be blocked and antitumor immune response can be restored by using by anti-PD-1 or anti-PD-L1 antibodies to block the binding of PD1 to PD- L1.

Targeting Strategy

Details

Phenotype

mRNA Expression Analysis

B-hCD274PD-L1-Mice-mrna-expression-analysis

 

The hPD-L1, but not mPD-L1, mRNA was detectable in splenocytes of the homozygous B-hPD-L1 mice.

Protein Expression Analysis

Cells in ascites from both wild type (WT)C57BL/6 and homozygous B-hPD-L1 mice were analyzed by flow cytometry. Mouse PD-L1 + B cells were detectable in the WT mice, while human PD-L1 + B cells were detectable in the homozygous B-hPD-L1 mice.

Splenocytes from both wild type (WT)C57BL/6 and homozygous B-hPD-L1 mice were analyzed by flow cytometry. Mouse PD-L1 + T cells were detectable in the WT mice, while human PD-L1 + T cells were detectable in the homozygous B-hPD-L1 mice.

Application

PD-L1(Tecentriq) Efficacy Evaluation

B-hCD274PD-L1-Mice-PD-L1-tecentriq-efficacy-evaluation

Modified murine colon cancer MC38 cells (mPD-L1 extracellular domain is replaced with the hPD-L1 extracellular domain, named as MC38-hPD-L1) were subcutaneously implanted into homozygous B-hPD-L1 mouse. Mice were divided into control and experimental groups (n=5) when the tumor size was about 100 mm3. Anti-hPD-L1 antibody Tencentiq (Atezolizumab) significantly inhibited tumor growth in the homozygous B-hPD-L1 mice at 3 different doses, suggesting that MC38-hPD-L1 cells in B-hPD-L1 mice is an effective model for in vivo hPD-L1 antibody efficacy study. (A) Tumor average volume ± SEM, (B) Mice average weight ± SEM.

References

1. Trends Mol Med. 2015 Jan;21(1):24-33. doi: 10.1016/j.molmed.2014.10.009. Epub 2014 Oct 30.
2. Proc Natl Acad Sci U S A. 2008 Feb 26;105(8):3011-6. doi: 10.1073/pnas.0712278105. Epub 2008 Feb 14.
3. Int Immunol. 2007 Jul;19(7):881-90. Epub 2007 Jul 2.

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